In the field of medical testing, coagulant separation gel blood collection tube is a commonly used blood collection tool that can quickly and accurately separate different components in the blood, providing pure and suitable samples for subsequent testing. The blood collection tube containing coagulant separation gel is mainly composed of a glass or plastic tube body, a rubber stopper, and internal coagulant and separation gel. The tube is usually marked with clear markings to facilitate accurate collection of the required amount of blood. Rubber plugs are used to seal blood collection tubes, preventing blood leakage and external contamination. What does the separated blood look like after being collected and treated with additives and centrifugation?

separation gel

The principle of action of blood collection vessel coagulant and separation gel

1. A coagulant is a chemical substance that can accelerate the process of blood clotting. When blood is collected into a blood collection tube containing a coagulant, the coagulant quickly interacts with coagulation factors and other components in the blood, initiating a coagulation cascade reaction and promoting the formation of stable blood clots in a short period of time.

2. Separation gel is a substance with a density between serum (or plasma) and blood cells. It has good chemical stability and will not undergo chemical reactions with components in the blood. During the centrifugation process, due to its density characteristics, the separation gel forms a clear physical barrier between serum (or plasma) and blood cells, achieving separation between the two under the action of the centrifuge.

Centrifugal process and separation of blood components

1. Centrifuge operation: After the blood has completed initial coagulation in the blood collection tube, it is necessary to put the blood collection tube into a centrifuge for centrifugation operation. The centrifuge generates strong centrifugal force through high-speed rotation, which causes the blood components in the blood collection tube to be arranged in layers according to density differences. When performing centrifugation, it is necessary to set the appropriate centrifugation speed and time according to the specifications of the blood collection tube, the performance of the centrifuge, and the requirements of the testing items.

2. Stratification of blood components: Under the action of centrifugal force, the separation of blood components in gel collection vessels by coagulants will exhibit significant stratification. Looking up from the bottom of the blood collection tube, the lower layer is the blood cell layer, mainly composed of red blood cells, white blood cells, and platelets. Due to their high density, blood cells reach the bottom of the blood collection tube under the action of centrifugal force. Above the blood cell layer is a separation gel layer, which maintains its original position during centrifugation, forming a clear barrier that separates blood cells from serum (or plasma). Further up is the serum (or plasma) layer, which is located above the separation gel due to its lower density than blood cells and greater density than the separation gel. Serum (or plasma) presents a clear and transparent state, containing various proteins, enzymes, metabolites, and other components, and is the sample required for most subsequent blood tests.

The use of separated blood samples

1. Purpose of serum (or plasma) samples: Serum (or plasma) samples are one of the commonly used types of samples in medical testing. They can be used for various blood biochemical tests, such as liver function testing (measuring the activity of enzymes such as alanine aminotransferase and aspartate aminotransferase, as well as the content of proteins such as bilirubin and albumin), kidney function testing (detecting the content of metabolites such as creatinine and urea nitrogen), blood lipid testing (measuring the content of lipid components such as cholesterol and triglycerides), immune testing (detecting the presence and content of various antibodies and antigens), etc. By analyzing these components in serum (or plasma) samples, one can understand the physiological state of the human body, monitoring effectiveness, etc.

2. The use of blood cell samples: Although blood cell samples are mainly used as a part of the separated gel collection vessels in coagulant separation, they also have their own uses. For example, by counting blood cells (such as red blood cell count, white blood cell count, platelet count), the basic composition of blood can be understood; Morphological observation of blood cells can reveal some characteristics of hematological diseases; In addition, some tests can be performed on blood cells, such as classification and counting of white blood cells, osmotic fragility tests of red blood cells, etc., to further understand the function and status of blood cells.

Product packaging

The coagulant separation gel blood collection vessel can quickly separate blood cells, separation gel, and serum (or plasma) from the blood, showing a clear layering phenomenon. These separated blood components each have different characteristics and uses, providing an important sample basis for medical testing and diagnosis. Hubei Xindesheng Material Technology Co., Ltd., as an important manufacturer of coagulants and separation gel, is cheap and can customize products and provide sample services. If you have purchasing intentions, please feel free to click on the website to inquire about details at any time!