In the experiment of acridine ester chemiluminescence, the commonly used instruments and equipment mainly include the following:

 

1. Fully automatic chemiluminescence analyzer/immunoassay analyzer

 

Function: These instruments are the core equipment for conducting chemiluminescence experiments, capable of automating the entire process of sample addition, dilution, oscillation, incubation, plate washing, reading, and result judgment.

 

characteristic:

 

The use of magnetic particle acridine ester direct chemiluminescence technology, such as Yahuilong's iStar 500 and iFlash 3000C fully automatic chemiluminescence analyzer, has the advantages of fast and concentrated photon release, low background luminescence, high signal-to-noise ratio, and high detection sensitivity.

 

Fast detection speed, such as iStar 500, which can achieve fast results in 11 minutes, and iFlash 3000C, which has a detection speed of up to 300 tests per hour and can produce results in 12 minutes.

 

Fully automated operation, freeing up manpower, easy to operate, with low requirements for personnel's professional background.

 

Acridine ester powder

 

2. 96 microporous plate (black)

 

Function: As a microcapacifying container, it holds many miniature test tubes for storing test samples or reagents.

 

characteristic:

 

Made of imported optically transparent pure polystyrene using special processes.

 

The bottom area is 0.32 square centimeters and the volume is 365 μ l, which can only add approximately 200 μ L.

 

Application: Widely used in numerous universities, research institutions, and biotechnology companies both domestically and internationally.

 

3. Acridine desalination column

 

Function: An instrument that separates mixed substances by molecular size by restricting the passage of molecules through porous media.

 

characteristic:

 

Mainly used for group separation, removal of high or low molecular weight pollutants, desalination, replacement of buffer solutions, and separation and collection of high-resolution biomolecule components based on differences in biological molecular weight.

 

The commonly used desalination medium is a bead shaped medium cross-linked and processed from 3-chloro-1,2-epoxypropane and pectin.

 

Different filler media affect their swelling degree and the selection of specific molecular sizes.

 

4. Spectrophotometer/Spectrometer

 

Function: Decompose complex light components into spectral lines and measure the luminescence or absorption intensity of specific wavelengths.

 

characteristic:

 

The principle of chemiluminescence spectrophotometer is similar to that of ordinary spectrophotometer, but there are differences. The light source of chemiluminescence spectrophotometer is the luminescence of the test liquid, and then the luminescence intensity is measured, usually requiring a more sensitive luminescence detector.

 

In chemiluminescence experiments, it is used to measure the luminescence intensity after the reaction of acridine ester, in order to determine the concentration of the analyte.

 

Product packaging

 

In summary, commonly used instruments and equipment in acridine ester chemiluminescence experiments include fully automatic chemiluminescence analyzers/immunoanalyzers, 96 microporous plates, acridine desalination columns, and photometers. These devices together form the chemiluminescence experimental system, ensuring the accuracy and stability of the experiment.

 

Desheng is a professional manufacturer of chemiluminescence reagents. Currently, the company produces and develops six types of acridine ester products, including NSP-DMAE-NHS, NSP-SA, NSP-SA-NHS, NSP-SA-ADH, DMAE-NHS, and Me DMAE-NHS. These products can be used for labeling different types of proteins, antibodies, and nucleic acids, bringing more convenience and possibilities to the field of biological detection.