In the daily practice of biomedical research, serum separation gel is an important experimental tool widely used in the processing and separation of blood samples. It separates serum from blood cell components through the physical barrier, providing high-quality samples for subsequent biochemical analysis, immunological testing, and more. However, in the crucial step of centrifugation, if the speed is set improperly, especially if it is too high, it may lead to hemolysis, which in turn affects the accuracy and reliability of the experimental results. This article aims to explore the effect of centrifugal speed on hemolysis phenomenon after blood collection with serum separation gel, and propose corresponding measures.

 

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The relationship between centrifugal speed and hemolysis phenomenon

 

Centrifugal speed is one of the key factors affecting serum separation efficiency. Appropriate rotation speed can ensure the separation of serum and blood cell components, while excessive rotation speed may cause a series of problems, among which hemolysis is particularly prominent. Hemolysis refers to the phenomenon in which red blood cells rupture when subjected to external forces (such as excessive centrifugal force), releasing intracellular components such as hemoglobin into the serum. These released components not only change the color of the serum, but more importantly, interfere with subsequent biochemical analysis, leading to deviations in experimental results.

 

Specifically, when the centrifugal speed exceeds the range that the serum separation gel collection tube can withstand, red blood cells will deform or even rupture under strong centrifugal force. After the rupture of red blood cells, hemoglobin and other cellular contents are rapidly released into the serum, forming hemolysis. After hemolysis, the serum sample appears dark red or reddish brown in color, and the concentration of biomarkers and enzymes inside it also undergoes significant changes, making it difficult to accurately reflect the true situation of the original blood sample.

 

The influence of hemolysis phenomenon on experimental results

 

The impact of hemolysis on experimental results is multifaceted. It can cause changes in the color and transparency of serum samples, affecting the accuracy of experimental observations. Secondly, substances such as hemoglobin released after hemolysis can interfere with the measurement of various biochemical indicators, such as electrolytes, enzymes, proteins, etc. These interfering factors may lead to deviations in experimental results or the occurrence of false positive/false negative results. In addition, hemolysis can also affect the storage stability of serum samples, accelerate the degradation or inactivation of certain biomarkers, thereby further reducing the reliability of experimental results.

 

Measures to prevent hemolysis

 

To avoid hemolysis caused by excessive centrifugal speed, we can take the following measures:

 

1. Strictly control the centrifugal speed and time:

 

When conducting serum separation, the centrifuge speed and time should be strictly set according to the instructions or experimental guidelines for the use of serum separation gel blood collection tubes. The recommended centrifugal speed is usually between 1800-2200 RPM (or the corresponding range of gravitational acceleration) for 8-10 minutes. Excessive rotational speed and prolonged time may increase the risk of hemolysis.

 

2. Ensure blood clotting:

 

Before centrifugation, it should be ensured that the blood sample has coagulated. Uncondensed blood is prone to issues such as residual fibrin during centrifugation, which can affect separation efficiency and increase the risk of hemolysis. Therefore, sufficient time should be given after blood collection to allow the blood to naturally clot.

 

3. Use appropriate centrifuges and centrifuge tubes:

 

Using a horizontal centrifuge for centrifugation operation can ensure that the sample maintains a balanced state during the centrifugation process, reducing the risk of hemolysis caused by vibration or imbalance. Meanwhile, centrifuge tubes that match the serum separation gel should be selected to ensure stability and safety during the centrifugation process.

 

4. Regularly inspect the centrifuge:

 

Regular maintenance and calibration of centrifuges are key to ensuring their stable and accurate operation. During use, attention should be paid to observing the operating status and changes in performance parameters of the centrifuge. If there are any abnormalities, they should be stopped in a timely manner and the cause checked.

 

5. Optimize the experimental process:

 

In addition to controlling the centrifugal speed and time, the risk of hemolysis can also be reduced by optimizing the experimental process. For example, during the blood collection process, be careful to avoid excessive oscillation or squeezing of the sample;; Take out the sample promptly after centrifugation and avoid placing it in the centrifuge for a long time.

 

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conclusion

 

In summary, the centrifugal speed has a significant impact on the hemolysis phenomenon of serum separation gel after blood collection. Excessive rotational speed increases the risk of hemolysis and affects the accuracy and reliability of experimental results. Therefore, when conducting serum separation, we must strictly control parameters such as centrifugation speed and time, and take a series of measures to reduce the risk of hemolysis. Only in this way can the accuracy and reliability of experimental results be ensured, providing strong support for biomedical research.

 

Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of serum separation gel. Since 2005, it has been engaged in the research and production of serum separation gel. In recent years, we have successfully upgraded to the fourth generation serum separation gel, with more stable indicators and more affordable prices. It is the choice of domestic and foreign customers. And it has its own large-scale factory and production line, with high daily output, which can meet the supply of bulk goods. If you have any purchasing needs, please feel free to contact us at any time. Looking forward to hearing from you!