In clinical medicine and laboratory research, blood collection tubes are important tools for collecting and storing blood samples. The selection, use, and technical requirements of their internal additives directly affect the quality of blood samples and the accuracy of subsequent test results. The following will provide a detailed explanation of the types, technical requirements, and usage precautions of blood collection tube additives to help everyone operate and use them more conveniently.

 

Serum separation gel

 

Types of blood collection tube additives

 

There are various types of blood collection tube additives, which can be classified into anticoagulants, coagulants, and serum separation gels based on their functions

 

1. Anticoagulants: mainly used to prevent blood clotting, ensure that blood samples remain in a flowing state after collection, and facilitate subsequent testing. Common anticoagulants include heparin sodium, heparin lithium, EDTA (ethylenediaminetetraacetic acid) dipotassium, tripotassium, and disodium. These anticoagulants achieve anticoagulant effects by inhibiting the formation of thrombin or platelet aggregation through different mechanisms.

 

2. Coagulants: Unlike anticoagulants, coagulants are mainly used to accelerate the process of blood clotting, allowing for rapid separation of serum and blood cells. Common coagulants include suspensions and coagulants, which stimulate platelets and clotting factors to promote blood clotting.

 

3. Serum separation gel: mainly used to separate serum or plasma from red blood cells for subsequent detection. The separation gel is mostly a polymer gel, which has a density between serum and red blood cells. It can quickly separate the two during centrifugation.

 

Technical requirements for blood collection tube additives

 

1. Accurate proportioning: The amount of additives used must be strictly in accordance with the instructions or laboratory standard operating procedures for accurate proportioning. Excessive or insufficient additives may affect the quality of blood samples and the accuracy of test results. For example, the amount of EDTA dipotassium added is usually 1.5-2.2mg per milliliter of blood. Excessive amounts may cause blood cell rupture, while insufficient amounts may not achieve anticoagulant effects.

 

2. Clean preparation: The preparation process of additives should ensure cleanliness. Dissolve powdered additives in deionized water or distilled water, and ensure that containers and tools are cleaned to avoid impurity contamination. For automated production equipment, it is also necessary to regularly clean the machines and conveying pipelines to ensure that there are no residues.

 

3. Proper mixing: After adding the additive to the blood sample, gently invert and shake the blood collection tube several times to ensure that the additive is thoroughly mixed with the blood. Insufficient mixing may lead to local coagulation or detection errors.

 

4. Time control: Some additives (such as non irreversible anticoagulants) have strict time requirements. For example, blood samples anticoagulated with heparin and EDTA should be tested as soon as possible after blood collection to avoid clotting or distorted test results.

 

5. Temperature management: Attention should be paid to temperature management during the storage and use of additives. Most blood collection tube additives need to be stored at room temperature (18-25 ℃) to avoid property changes caused by high or low temperatures. For additives that require heating treatment (such as separation gels with increased viscosity at low temperatures), the heating temperature and time should be strictly controlled.

 

Storage conditions

 

1. Temperature control: Most blood collection tube additives need to be stored at room temperature to avoid property changes caused by high or low temperatures. For additives that require refrigeration, they should be stored strictly according to the instructions.

 

2. Avoid light storage: Blood collection tube additives should be kept away from direct sunlight to prevent changes in their performance. When storing, try to choose a dark and dark place.

 

3. Humidity control: Excessive or insufficient humidity can affect the stability of blood collection tube additives, and excessively humid environments should be avoided. Additive raw material powders are prone to moisture absorption and agglomeration in excessively humid environments.

 

4. Sealed storage: Additives should be sealed and stored to avoid oxidation or deterioration caused by prolonged contact with air. For additives that have already been activated, they should be used up as soon as possible and the remaining parts should be properly stored.

 

The technical requirements for blood collection tube additives involve multiple aspects, including accurate ratio, appropriate mixing, time control, temperature management, etc. Only by strictly adhering to these requirements can we ensure the quality of blood samples more ideally.

 

Product packaging

 

As a manufacturer of blood collection tube additives, Desheng sells products such as serum separation gel, EDTA dipotassium, heparin sodium, heparin lithium, coagulants, etc. at a low price and with stable spot supply. At present, we have established cooperative relationships with multiple blood collection tube companies. If you are interested, please feel free to click on the website to inquire about details and purchase at any time!