The key role of EDTA dipotassium and serum separation gel products in blood pretreatment

Release time:

2024-09-21


The pre-treatment process of blood is a crucial step in ensuring the accuracy and stability of blood samples during subsequent testing. EDTA dipotassium, serum separation gel, and other blood collection tube additives play a crucial role in blood pretreatment. The following is a detailed analysis of this process.

 

EDTA dipotassium powder

 

1、 Test tube preparation

 

Pre use inspection of test tubes: Check disposable test tubes before use to ensure their quality is qualified and provide a good foundation for subsequent silicification treatment.

 

Silicification treatment: Silicification is a crucial step in the preparation of test tubes. Silicifying agent is a product with good chemical and physiological inertness, which can make the inner wall of the test tube smooth. The main purpose of this step is to prevent blood cells in the blood from rupturing when they come into contact with the rough inner wall of the test tube, thereby avoiding hemolysis. Hemolysis not only destroys the integrity of blood samples, but may also interfere with subsequent test results. Silicification treatment can also avoid the activation effect of test tubes on cells or blood, and avoid the influence of enzyme-linked reactions.

 

2、 Addition of additives

 

Coagulants or anticoagulants: After the silicification treatment is completed, an appropriate amount of coagulants or anticoagulants need to be added to the test tube according to the requirements of the inspection items. Coagulants can accelerate the coagulation process of blood and are suitable for testing items that require serum or plasma; Anticoagulants can prevent blood clotting, maintain the morphology of blood cells, and ensure accurate counting, making them suitable for testing projects that require whole blood or specific blood cells.

 

EDTA dipotassium or serum separation gel: For certain specific testing items, such as blood routine tests or items that require separation of serum and plasma, additives such as EDTA dipotassium or serum separation gel will also be added to the test tube. EDTA dipotassium is a commonly used anticoagulant that can prevent blood clotting; Serum separation gel can separate serum (or plasma) from blood cells after blood coagulation, facilitating subsequent testing operations.

 

3、 Blood collection and preservation

 

Blood collection: After the test tube is prepared and the corresponding additives are added, blood collection can be carried out. The collected blood sample needs to be quickly injected into a test tube and gently inverted several times to ensure that the blood and additives are fully mixed.

 

Storage and transportation: The collected blood samples need to be stored and transported under appropriate conditions. For samples that require maintaining blood flow (such as those with added anticoagulants), they usually need to be stored at low temperatures; For samples that have already solidified (such as those with added coagulants), they can be stored at room temperature for a short period of time. During transportation, it is necessary to avoid severe vibrations and temperature changes to ensure the stability of the sample.

 

Product packaging

 

In summary, the pre-treatment process of blood includes preparation of test tubes, blood collection tube additive of additives (such as coagulants, anticoagulants, EDTA dipotassium or serum separation gels), and collection, storage, and transportation of blood samples. These steps together ensure the accuracy and stability of the blood sample in subsequent testing. Desheng can provide a full range of blood collection tube additive products, such as serum separation gel, coagulants (suspension coagulants, coagulants powder), anticoagulants (EDTA dipotassium, EDTA tripotassium), silicification agents, etc. Welcome to visit our factory!