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The development history of the chromogenic substrate DAOS reagent

Release time:

2020-07-04

The color development substrate DAOS reagent belongs to a kind of Trinder’s reagent, CAS number: 83777-30-4, and is a very important raw material in the biochemical reagent kit. The reaction of the color reagent as an indicator can be directly measured with a spectrophotometer, and can even be observed with the naked eye. It is an earlier widely used detection method, but in the beginning, there was no such reagent as DAOS, let us take a look Its development.

Linkase Immunochromogenic Substrate Reagent

Color-developing reagent is a reagent that produces color or changes in color after biochemical reaction to achieve the purpose of experimental detection and diagnosis. There are many types of reagents. The most common pH test paper used to detect the pH is a dry chemical detection method realized by color development. Color-developing reagents are both inorganic and organic, and enzyme-linked immunochromatographic substrate reagents with enzymes are the most commonly used in biochemical detection. Because the enzymatic reaction is very rapid, with high sensitivity and strong specificity, it is very conducive to the rapid detection and data analysis of the detection instrument.

Colourful color reagents

Development of chromogenic substrate reagents

There are many kinds of coloring substrate reagents. The first used were phenol, aniline, DAB, but these sensitivity is not high enough, and it has certain carcinogenicity. Nowadays commonly used are TMB in ELISA kit and each in biochemical kit A new type of Trinder's reagent. DAOS is a kind of Trinder's reagent. The reaction principle is similar to TMB. They are derivatives of phenol or aniline. In the presence of peroxidase POD, they can be directly oxidized or coupled by hydrogen peroxide to produce color. Reaction, then detect, analyze and get the result under the spectrophotometer.

Types of new Trinder’s chromogenic substrate reagents

DAOS color substrate

There are a total of 10 new Trinder's reagents, all of which are highly water-soluble aniline sodium salt derivatives. Each color product has a different absorption wavelength and molar absorbance. The experiment can choose different ones according to different sensitivity and interference conditions. Color-developing substrates, of course, have different companies' kits for detecting uniform indicators. Different color-developing substrates are used. For a certain test index, some color-developing substrates are not unique. These chromogenic substrates form the basis for enzymatic determination of glucose, cholesterol, triglycerides, and uric acid.

The chromogenic substrate DAOS is usually used in the low-density lipoprotein detection kit of the blood lipid detection series. The absorption maximum wavelength of the chromogenic product is 593nm, which is slightly lower than that of MAOS, but its reaction can be maintained for a long time without fading. The interference of other substances in other samples is small, and the accuracy of the test results is high. Desheng has rich experience in the synthesis and development of color-developing substrates. The products cover a variety of color-developing substrates and corresponding enzyme preparations. Customers are welcome to cooperate!