Enzyme colorimetry, as a classic and constantly evolving technique, plays a crucial role in clinical laboratories and scientific research due to its intuitive and sensitive characteristics. With the continuous development of new chromogenic substrates such as TOOS, MAOS, ADPS, etc., the application scope and detection accuracy of enzyme colorimetric methods have been significantly improved, providing more possibilities for rapid detection of biochemical indicators.

MAOS reagent

The absorption wavelength of the chromogenic substrate does not directly correspond to the color presented by its reaction product, but follows the principle of complementary color. For example, although MAOS has strong absorption in the 630nm orange red region, it appears blue-green after the reaction. In addition, the selection of chromogenic substrates also needs to consider the potential interference of other components in the sample. For example, MAOS, due to its high absorption wavelength, can effectively avoid non-specific absorption in complex biological samples, making it an ideal choice for high-sensitivity detection. So why can high absorption wavelength color reagents effectively avoid interference? There are several reasons for this.

Reduce spectral overlap interference: In biochemical testing, samples may contain multiple components that may absorb light at specific wavelengths, leading to interference in the detection signal. The absorption wavelength of MAOS is as high as 630 nanometers, located in the orange red region, which far exceeds the absorption range of many conventional biomolecules and interfering substances (such as bilirubin in blood, which has an absorption wavelength of 380-530 nanometers). Therefore, choosing to detect at such a high wavelength can significantly reduce the absorption interference of other sample components, improve the specificity and accuracy of detection.

Enhanced signal-to-noise ratio: Due to less non-specific absorption in high wavelength regions, the signal generated by MAOS is more prominent compared to background noise, which means that even in complex samples, the signal of the target product is easier to distinguish. This enhances the sensitivity and reliability of the detection.

Advantages of instrument detection: Modern detection equipment such as spectrophotometers typically have better performance in longer wavelength regions, including higher sensitivity and lower levels of stray light, which helps to obtain clear and accurate measurement results at wavelengths such as 630 nanometers.

Complementary color principle: According to the complementary color principle in optics, the absorbed light and its displayed color complement each other. MAOS has strong absorption at 630nm (orange red light), and the color of the reaction product of the substrate will show blue-green, which is the complementary color of 630nm light.

Product packaging

Specific design to reduce interference: Many common interfering substances, such as pigments and metabolites in biological fluids, often have absorption spectra concentrated in the low wavelength region of visible light. Choosing high wavelength color reagents such as MAOS can avoid the absorption peaks of these common interfering substances and reduce their impact on the detection results.

In the field of research and production of chromogenic substrates, Desheng is at the forefront of the industry with its profound technical accumulation and keen market insight. In addition to the new Trinder's reagent, there are also DA64, DA67, etc., which have higher absorption wavelengths than MAOS. Desheng continues to innovate and strive to provide more sensitive chromogenic substrates, further expanding the boundaries of biological labeling and detection.