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The application scope and characteristics of BIS-TRIS buffer

Release time:

2024-07-06

BIS-TRIS buffer, as a zwitterionic biological buffer, plays an important role in various fields such as cell culture media, chromatography, electrophoresis, and protein purification. It has a wide pH buffering range (5.8-7.2) and can maintain the acid-base balance of the system under different conditions. BIS-TRIS has become an indispensable assistant for researchers due to its excellent performance and wide range of applications. The following are its main application areas and characteristics.

BIS-TRIS buffer

Application scope

Cell culture medium: BIS-TRIS buffer plays an important role in cell culture, providing a stable pH environment for cells and ensuring that they are not affected by pH fluctuations during growth and reproduction.

Chromatography: In chromatography, BIS-TRIS buffer can maintain pH balance between mobile and stationary phases, ensuring the integrity of the sample's structure and function during separation.

Electrophoresis: BIS-TRIS is a popular electrophoresis buffer suitable for various types of electrophoresis. Together with ACES buffer, it can be used as the running buffer of gel electrophoresis (BIS-TRIS/ACES) to provide a stable pH environment for the separation and analysis of biological macromolecules such as proteins and DNA.

Protein purification: During the protein purification process, BIS-TRIS buffer can help remove impurities and improve protein purity. At the same time, it can also maintain the stability and activity of the protein, ensuring that the purified protein has good biological functions.

In addition to the aforementioned fields, BIS-TRIS has also been reported to have applications in anion exchange chromatography, NMR spectroscopy, and X-ray crystallography. Among these complex and precise technologies, BIS-TRIS provides reliable support for researchers with its excellent performance and stability. BIS-TRIS is capable of separating and purifying complex samples, as well as analyzing the structure and function of biological macromolecules, and can leverage its advantages.

Matters needing attention

Not applicable for BCA analysis: When conducting BCA analysis, BIS-TRIS buffer should be avoided as it may affect the accuracy of the analysis results.

Product packaging

Interaction with human liver fatty acid binding protein (FAB): BIS-TRIS buffer interacts with human liver fatty acid binding protein (FAB), which may affect the dynamic properties of the protein. Therefore, in studies involving FAB, caution should be exercised when using BIS-TRIS buffer.

In the field of scientific research, choosing a reliable BIS-TRIS buffer is crucial. As a professional manufacturer and supplier of biological buffering agents, Desheng is committed to providing reliable BIS-TRIS products for scientific researchers. We strictly control product quality to ensure that every batch of products meets standards and requirements. At the same time, we also provide personalized customization services to meet the needs of different scientific research projects.