Serum separation tube is a commonly used tool in clinical laboratories for separating serum from whole blood. Serum is a pale yellow transparent liquid separated from blood after coagulation. It does not contain fibrinogen, but contains a large amount of proteins, antibodies, and other bioactive substances. It is a variety of physiological and pathological indicators and has important value for clinical diagnosis and disease monitoring. The appropriate rotational speed is a key factor in ensuring the effectiveness of serum separation when using serum separation tubes for serum collection.

serum separation

The importance of rotating speed of serum separation tubes

The speed of the centrifuge is a key parameter in the serum separation process. The appropriate rotational speed can ensure that red and white blood cells in the blood effectively settle at the bottom of the test tube, while serum is clearly separated in the upper layer. Low rotational speed may lead to incomplete sedimentation of red and white blood cells, while high rotational speed may result in the appearance of small red or white blood cell fragments in the serum, thereby affecting subsequent experimental results.

Working principle of serum separation hose

Serum separation tube is a specially designed test tube with a special layer of separation gel coated inside. This layer of separation gel can form a clear interface during centrifugation, separating serum from red blood cells. When the blood sample is placed in a serum separation gel tube and subjected to appropriate centrifugation, red blood cells will settle at the bottom of the test tube, while the serum will be separated from these cell components through the separation gel layer and collected in the upper part of the test tube.

Speed settings for different types of centrifuges

Different types of centrifuges have different higher rotational speeds and centrifugal forces. Common centrifuges include desktop centrifuges, high-speed centrifuges, etc. When selecting a centrifuge and setting the speed, it is necessary to determine based on the type and specifications of the centrifuge tube.

(1) Desktop centrifuge: For general serum separation tubes, a desktop centrifuge is usually used for centrifugation. The common speed setting is between 3000-5000 rpm. During use, the appropriate speed can be selected according to the centrifuge manual or laboratory standard operating procedures.

(2) High speed centrifuge: Some experiments may require higher speeds, in which case a high-speed centrifuge is required. The high-speed centrifuge has a high rotational speed, which can reach tens of thousands of rpm. In this case, it is necessary to set the appropriate rotational speed based on specific experimental requirements and the specifications of the centrifuge tube.

Precautions in the experiment

When conducting serum separation experiments, in addition to selecting the appropriate rotational speed, the following precautions should also be taken:

1. Sample size: The amount of sample will affect the serum separation effect, and it is important to collect according to the specified amount in the test tube.

2. The placement of centrifuge tubes: There are usually slots in different positions in the rotor of the centrifuge, and the centrifuge tubes should be placed in the corresponding positions to ensure balance during the centrifugation process.

3. The combination of rotational speed and time: The appropriate rotational speed and appropriate centrifugation time are the key to ensuring sufficient serum separation, which needs to be determined according to the needs of the experiment, generally within 3 minutes.

Product packaging

When using serum separation tubes for serum collection, selecting the appropriate rotational speed is a key step to ensure experimental results. Before conducting relevant experiments, operators should carefully understand the performance parameters of the centrifuge and choose the appropriate speed according to the experimental requirements to ensure high-quality separation of serum. As a manufacturer of blood collection additives, Desheng can provide high-quality separation gel with good flipping performance and accurate specific gravity. Adding it to blood collection vessels can effectively complete blood separation and collect high-quality serum samples. If you have any relevant intentions, please click on the website to inquire about details and purchase!