What is the reason for the failure or poor flipping of serum separation gel?

Release time:

2023-12-20


Non flipping or poor flipping of serum separation gel is a common problem in clinical laboratories and biomedical research. Understanding the reasons for non flipping or poor flipping of serum separation gel and taking corresponding measures to solve it is of great significance for improving the accuracy of experimental results and work efficiency.

The failure or poor flipping of serum separation gel may be caused by various factors. Among them, the thixotropy of serum separation gel is one of the important factors. Thixotropy refers to the rearrangement of molecular chains inside a serum separation gel under external forces, resulting in changes in viscosity and fluidity. If the thixotropy of serum separation gel is poor, it will cause the serum separation gel to not flip or flip poorly. This may be due to improper formula or process of serum separation gel, or due to storage time being too long or temperature being too high.

In addition to the thixotropy of serum separation gel, centrifugal conditions are also an important factor affecting the flipping of serum separation gel. If the centrifugation speed is too slow or the time is too short, the serum separation gel may not be able to fully flip, resulting in incomplete separation of serum and blood cells. In addition, factors such as the placement of the centrifuge tube, the balance of the centrifuge shaft, and the stability of the rotational speed can also affect the flipping effect of the serum separation gel.

In addition, improper treatment of the inner wall of the test tube is also one of the factors that lead to the failure or poor flipping of the serum separation gel. Before serum separation, it is necessary to treat the inner wall of the test tube to avoid serum adsorption on the inner wall. If the inner wall of the test tube is not properly treated, such as incomplete cleaning or disinfection, it can lead to the inability or poor flipping of the serum separation gel.

In addition to the above factors, quality issues with blood samples are also one of the factors that lead to the failure or poor flipping of serum separation gel. If there are hemolysis, clots, and other issues in the blood sample, it can cause the serum separation gel to fail to flip or flip poorly.

To solve the problem of non flipping or poor flipping of serum separation gel, the following measures can be taken:

1. Optimize the formula and process of serum separation gel to improve its thixotropy performance. This can be achieved by changing the formula of serum separation gel, adjusting the proportion of each component, and improving the production process. After optimizing the formula and process of serum separation gel, it can better play a role in the centrifugation process and improve the separation effect of serum and blood cells.

2. Strictly control the centrifugation conditions to ensure that the centrifugation speed and time are appropriate, to ensure that the serum separation gel is fully flipped. Before centrifugation, it is necessary to choose the appropriate centrifugation speed and time according to the experimental requirements to ensure that the serum separation gel can fully flip and separate the serum. At the same time, it is also necessary to pay attention to the placement position of the centrifuge tube and the balance of the centrifuge shaft to ensure the stability and good effect of the centrifugation process.

3. Properly treat the inner wall of the test tube, such as cleaning or disinfecting, to avoid serum adsorption on the inner wall. Before serum separation, it is necessary to treat the inner wall of the test tube, such as using specialized cleaning agents or disinfectants for cleaning or disinfection, to remove impurities and pollutants and avoid serum adsorption on the inner wall. At the same time, attention should also be paid to the cleaning and maintenance of test tubes to maintain their cleanliness and hygiene.

4. Strictly control the quality of blood samples and avoid using blood samples with issues such as hemolysis and clotting. When collecting blood samples, it is necessary to pay attention to the collection methods and techniques to avoid problems such as hemolysis or clots caused by excessive compression or vibration. At the same time, strict adherence to operating procedures and hygiene requirements is also necessary when storing and handling blood samples to ensure their quality and safety.

Understanding the reasons for non flipping or poor flipping of serum separation gel and taking corresponding measures to solve it is the key to ensuring the accuracy of experimental results and work efficiency. At the same time, it is necessary to strengthen the quality control and management of the laboratory to ensure the standardization and normalization of the experimental process.

Desheng is not only a high-quality manufacturer of serum separation gel, but also a professional supplier of blood collection additives. In addition to striving to improve quality, it is also committed to providing laboratory personnel with a set of clinical blood sample solutions, including operating standards in the transportation, storage, blood collection, and centrifugation processes of blood collection vessels, truly achieving first-class quality and service.