Trihydroxymethyl aminomethane powder
Trihydroxymethyl aminomethane is a commonly used buffer that has good buffering capacity and stability, and can maintain the pH value of the solution within a certain range. It is widely used in fields such as biochemistry, molecular biology, cell biology, and industrial production. What are the characteristics and uses of Tris buffer, which was originally produced as white crystalline powder? Below, we will focus on introducing it to everyone.
1、 Characteristics of Trihydroxymethyl Aminomethane Powder
1. Chemical properties: Trihydroxymethylaminomethane powder has high stability at room temperature due to its weak alkaline solution. When the surrounding temperature is below 25 ℃, its Pka is 8.1. According to the buffer range, its effective buffer pH value is between 7.0 and 9.2.
2. Solubility: Trihydroxymethylaminomethane powder is soluble in water, ethanol, and ethyl acetate. It is generally dissolved in deionized water because it dissolves slowly with ethyl acetate and has a corrosive effect on copper and aluminum. At the same time, note that its solubility will increase with the increase of temperature, so it is necessary to adjust the concentration and pH value of the solution according to requirements in the experiment.
3. Buffering capacity: Prepared buffering agents can provide stable buffering capacity within different pH ranges, typically having the best buffering effect between pH 7-9. They have been used in many biological experiments to maintain pH stability in organisms.
Product level AR analysis pure level
Special indicators of manufacturer service can be customized and sample tested
Inventory spot sales
Specification size 500g, 25kg/barrel
Storage method: Keep away from light and sealed at room temperature, and do not expose to air
2、 Use of Trihydroxymethyl Aminomethane
1. Protein electrophoresis: Tris buffer is widely used in protein electrophoresis experiments to maintain the pH stability of gel and electrode solution in gel electrophoresis system, so as to ensure the stable operation of gel electrophoresis.
2. DNA/RNA gel electrophoresis: it is used in DNA/RNA gel electrophoresis experiments to prepare agarose gel and electrode buffer to ensure the stability of DNA/RNA migration in electric field.
3. Cell culture: Tris buffer can be used in buffer solutions for cell culture to help maintain the stability of the cell culture environment and promote cell growth and proliferation.
4. Pharmaceutical Intermediates: In the process of drug development, Tris can be used as pharmaceutical intermediates to help adjust the pH and stability of drugs.
5. Biochemical experiments: Used as a buffer for reaction systems in many biochemical experiments to help maintain the stability and efficiency of reactions.
Trihydroxymethylaminomethane is a commonly used biological buffer with good chemical properties and solubility. Due to its stable buffering ability and versatility, it plays an important role in scientific research and experimental applications. As an advantageous manufacturer of buffering agents, Desheng has a purity of up to 99% and is commonly used in various experimental studies to achieve stability. It also offers discounted factory prices. If you are interested, please feel free to contact us for purchase!
Trihydroxymethylaminomethane is an important laboratory reagent widely used in fields such as molecular biology and biochemistry. To ensure its quality and purity, a series of tests are required. This article provides measurement methods for appearance, content, solubility, drying weight loss, pH value, melting point, and UV absorbance. These methods help to evaluate the quality and purity of trimethylaminomethane.