Differences between Tris base and Tris hcl
Tris and Tris hcl are well-known biological buffering agents, with the chemical names Trihydroxymethylaminomethane and Trihydroxymethylaminomethane hydrochloride. Tris base, a chemical substance that seems unfamiliar, is related to the two chemicals mentioned above?
Tris base powder
Tris base is actually a relatively complete English alias for tris, which means "base" in Chinese, indicating that tris is an alkaline substance, which is consistent with the acidity and alkalinity of tris aqueous solutions. This means that tris and tris base are two different names for the same substance.
Tris base and Tris hcl are two different biological buffering agents, which are introduced one by one below:
1. Different pH values
The pH value of Tris base aqueous solution at 25 ℃ is between 10.5 and 12.0, indicating an alkaline solution; Tris hcl is an acidic solution with a pH value between 2.5 and 4.0 in aqueous solution at 25 ℃. Due to their different pH values and corresponding buffer ranges, the applicable biochemical experiments are also different, and tris hcl is prepared by configuring tris base and hydrochloric acid.
2. Different scope of application
Tris base can be used for protein crystal growth under different acid-base conditions, as well as for pharmaceutical intermediates and the preparation of active agents; Tris HCl is widely used in nucleic acid and protein solvents, and can also form electrophoretic solutions with glycine. It can also be developed as an adjunct for use in some pharmaceutical products.
3. Differences in other aspects
Tris base and Tris hcl have different acidity coefficients, molecular formula, molecular weight, CAS number, etc. at room temperature, except for different pH values and buffer ranges. Although their appearance is white crystalline powder, their detailed information is still easily recognizable upon careful observation.
packing of products
Desheng specializes in the production of biological buffers , with stable production processes and small batch differences. The purity of the buffer produced is as high as 99%, and the appearance is white crystal powder, making it easy to store and handle. And the company has an independent production workshop and advanced production equipment. The stock of buffer agents is sufficient and the variety is complete. If you are interested in purchasing, please click on the official website to check!
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