Analytical pure N - (2-acetylamino) -2-aminoethanesulfonic acid (ACES) buffer raw material
In modern life science research, analytical pure buffering agents play a crucial role in experiments, providing researchers with a stable experimental environment to ensure the accuracy and reliability of experimental results. N - (2-acetylamino) -2-aminoethanesulfonic acid, commonly known as ACES buffer, is a commonly used buffer and has been widely used in scientific research due to its unique properties.
1、 Characteristics and composition
The CAS number of ACES is 7365-82-4, with a pKa value range of 6.1-7.5, which is suitable for serving as an acid-base regulator in reaction solutions with pH values of 6.6-7.0. This means that within this pH range, ACES can effectively maintain the acid-base balance of the solution. It generally contains components such as sodium hydroxide and sodium chloride, which can regulate the ion strength and ion balance of the buffer solution. However, it should be noted that although ACES is an excellent buffer, its buffering performance is greatly affected by temperature. Therefore, temperature changes need to be taken into account in experimental design.
2、 Application field
1. Cell culture: ACES buffer, after strict filtration and sterilization treatment, can be directly added to the cell culture medium to regulate the acid-base environment of the medium and provide a suitable growth environment for cells. Especially in some special cell culture media, such as Legionella culture medium and Changchun flower hairy root culture medium, ACES can maintain normal cell growth.
2. Enzyme activity research: Due to its pH range being consistent with the activity range of many enzymes, it is often used for enzyme activity research, which can stabilize the experimental environment and ensure the effective catalysis of enzymes.
3. Nucleic acid research: In nucleic acid research, ACES buffer is more suitable for identifying nucleic acids with larger molecular weights, and can maintain stable experimental conditions compared to other buffer agents.
4. Glucosidase research and X-ray crystallography: ACES buffer has shown excellent performance in glucosidase research and X-ray crystallography of aldehyde dehydrogenation complexes, providing reliable data support for these areas of research.
N - (2-acetylamino) -2-aminoethanesulfonic acid (ACES) buffer has been widely used in life science research due to its unique characteristics, such as temperature sensitivity and applicability. For researchers, understanding the characteristics of ACES buffer raw materials can better provide a stable experimental environment for experiments and ensure the reliability of experimental results.
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