The absorption wavelength and molar absorption intensity of chromogenic substrate MAOS
In biochemical analysis and experiments, chromogenic substrates play a crucial role, as they can convert the information of the tested molecules into visible color signals through chromogenic reactions, thereby achieving analysis and detection. For a chromogenic substrate, its absorption wavelength and molar absorption intensity are important indicators to measure its performance and applicability. MAOS (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline sodium salt), as a chromogenic substrate, has a series of unique characteristics, which has attracted much attention in the field of biochemical analysis.
1、 The particularity of absorption wavelength
One of the major characteristics of MAOS is that the absorption wavelength of its oxidized products in the ultraviolet region significantly exceeds the absorption wavelength of many ordinary color reagents. Generally speaking, the products of color reagents have a larger absorption wavelength in the visible light region, but this may result in absorption wavelengths similar to some components in the sample to be tested, which can interfere with the detection results. The larger absorption wavelength of MAOS products is quite high at 630nm in the ultraviolet region, which provides good conditions for reducing interference, especially for biochemical detection projects that require highly accurate values. This characteristic of MAOS can significantly reduce interference, improve detection accuracy and reliability.
2、 The influence of molar absorption intensity
Molar absorption intensity is an important indicator of color reaction, which is directly proportional to the concentration of the product. As a chromogenic substrate, MAOS has a high molar absorbance, which means that it can generate strong signals in the chromogenic reaction, thereby improving the sensitivity of detection. Under high molar absorbance, even if the concentration of the analyte is low, a clear color signal can be obtained, providing analysts with a wider range of application space.
3、 Precautions and Safety
Although MAOS performs well in many aspects, there are still some details to pay attention to when using it. Firstly, due to its high molar absorbance, the color reaction may be relatively rapid, so the detection time should not be too long. Secondly, although MAOS is relatively safe, strict adherence to safety operating procedures is still necessary during the experiment to avoid contact with skin and eyes.
The chromogenic substrate MAOS, characterized by its product absorption wavelength in the ultraviolet region, high molar absorption intensity, and high sensitivity, performs well in detection projects that require high accuracy and low interference, providing researchers with a reliable and efficient analysis tool.
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