How long can Luminol solution be stored at room temperature?
Luminol is a common chemiluminescence reagent, which is widely used in biomedicine, molecular biology, immunology and other fields. Its luminescence reaction plays a crucial role in many experimental and diagnostic applications, such as enzyme linked immunosorbent assay (ELISA). However, for scientific research and laboratory work, the storage time of Luminol solution has always been a matter of concern. Now let's talk about the storage life of Luminol solution at room temperature in detail.
1、 Chemical characteristics of Luminol
Luminol is a chemiluminescent reagent, its chemical name is 3-amino-phenylenedicarbohydrazide, and its original character is brown yellow powder. In the luminescence experiment, the prepared Luminol solution can catalyze the production of active oxygen with Catalase and stimulate the fluorescence luminescence reaction to achieve the purpose of detecting target molecules.
2、 Stability of Luminol Solution
Luminol solution is relatively stable after preparation, but its stability is sometimes affected by many factors, such as light, oxygen, temperature and pH value. For solutions, it is generally crucial to avoid exposure to light and air, as both light and oxygen can promote oxidation of the solution, leading to a decrease in its luminescence intensity.
3、 Storage time of Luminol solution at room temperature
The storage time of Luminol solution is not long under normal temperature conditions, but it can be sealed and stored within 1 week under cold storage conditions. Although there is a storage period, in order to ensure the accuracy of experimental results, it is usually recommended to use immediately or use it in a short time. If the time is long or the solution changes during storage, it is still necessary to prepare a new Luminol solution before each experiment to avoid affecting the experiment.
4、 Storage method of Luminol solution
Although the storage time of Luminol solution is limited, some storage details should be paid special attention to:
1. Store in a dark place: Avoid exposure of Luminol solution to light, which can slow down its oxidation reaction and extend the storage time.
2. Avoid contact with oxygen: When storing a solution, try to avoid oxygen coming into contact with the solution. This can be achieved by tightening the bottle cap or sealing it with nitrogen gas.
3. Cold storage: Although the storage time of Luminol solution at room temperature is limited, cold storage (4 ° C) can slightly extend its stability.
Luminol solution is a common chemiluminescence reagent, which is used in many biological experiments and diagnostic applications. However, due to the influence of factors such as light and oxygen on its stability, in order to ensure the accuracy of experimental results, it is recommended to use it immediately and observe the solution for any deterioration at any time. As a professional manufacturer of luminescent reagents, Desheng can provide reagents that are sensitive, affordable, and have a complete range of products. If you are interested, please feel free to contact us for purchase at any time!
When it comes to the chemiluminescent reagent Luminol, it is not unfamiliar to everyone and is commonly used in the field of criminal investigation to detect blood stains. Researchers have found that the principle of luminol luminescence can be used to detect specific substances in saliva, such as proteins, DNA, and related indicators of microorganisms, in order to determine physical conditions.