What is the solubility and dissolution method of potassium ethylenediaminetetraacetic acid?
Dipotassium ethylenediaminetetraacetic acid is a white crystalline powder with no taste or odor, and high solubility. Therefore, it is often prepared into a solution and added to a vacuum test tube as a blood anticoagulant treatment reagent. Although most of the finished products produced in the market have the same characteristics, their manufacturing processes vary, and the solubility and dissolution methods between the finished products are also different. If not paid attention, it will have an impact on the test results. So next, we will specifically introduce the solubility and dissolution method of EDTA K2.
1、 Solubility of potassium ethylenediaminetetraacetic acid
Due to its powder like nature, it is soluble in water and easily hygroscopic. At room temperature of 25 degrees, its solubility in water is ≥ 60% (g/ml), and its concentration is equivalent to 2.3mg/ml. At this time, the pH value of the potassium ethylenediaminetetraacetate solution is between 3.0 and 3.5. If the pH value of the solution is adjusted to increase its solubility, it will also increase accordingly. Generally, the solubility of potassium ethylenediaminetetraacetate is higher than that of disodium ethylenediaminetetraacetate, and its anticoagulant effect is more comprehensive. Therefore, it is also an ideal anticoagulant recognized in international medicine.
2、 Dissolution method of potassium ethylenediaminetetraacetic acid
The main dissolution method is to add deionized water. Although the dissolution method is relatively simple, attention should also be paid to details. If residual powder is seen on the inner wall of the beaker used, it needs to be slowly flushed into the bottle using a washing bottle. Note that the solution container needs to be cleaned thoroughly and should not contain impurities. And during dissolution, attention should be paid to controlling the addition of aqueous solution, and a certain cleaning volume should be left for the equipment used, otherwise it may affect the concentration level.
3、 Concentration of potassium ethylenediaminetetraacetic acid
The concentration of potassium ethylenediaminetetraacetic acid added to the test tube should not be too high or too low. Its suitable range is between 1.5 and 2.2mg per milliliter of blood, in order to ensure the accuracy of blood test results. If the blood volume is insufficient and the concentration is as high as 2.5mg/ml, the osmotic pressure will become higher, the blood cell components will break, and the blood samples collected will be meaningless.
So not only should attention be paid to dissolution, but also to the added concentration after dissolution. As a professional supplier of potassium ethylenediaminetetraacetate, Desheng's product quality has always been recognized by the market. The company has conducted overall tests on its main content, pH value, solubility, and appearance, and can only be sold after passing the standards. Recently, more and more customers have come to order potassium ethylenediaminetetraacetate. If you are also interested, please click on the website to inquire and purchase!
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