Application of Good's Buffer TES
TES buffer is a kind of Good's buffer series, which is a zwitterionic buffer for biochemistry and molecular biology, and it is also part of the Tris buffer family, because it is synthesized by Tris + EDTA + SDS, so it is called TES buffer. The pKa of TES at 25°C is just below physiological pH (7.4), so it has an effective pH range of 6.8 – 8.2 and its dissociation constant changes only slightly with temperature.
It is used in various protein assays, micellar electrokinetic chromatography and testing egg yolk buffer media. Like most neutral pH, amine-based buffers, TES forms a complex with DNA. It has a greater effect on restriction enzyme kinetics than HEPES, but less than Tris. It also forms complexes with Cu(II) ions. TES can affect mRNA expression and embryonic development, so it is also suitable for bicinchoninic acid (BCA) assays. Below are some of the main uses of TES buffers, let's take a look!
Uses of TES buffers:
1. As a cell washing buffer and a solution for DNA precipitation and extraction;
2. As an enzyme assay buffer;
3. For gel filtration and affinity chromatography;
4. In personal care and cosmetic applications, TES can protect the active ingredients in the formula from being degraded;
5. It is suitable for the separation of hemoglobin;
6. It can protect the stability of hemoglobin during freeze-dried storage;
7, 2-D gel electrophoresis as the catholyte in isoelectric focusing electrophoresis (IEF).
Hubei Xindesheng Material Technology Co., Ltd. is a source manufacturer of biological buffers. It not only has more than ten years of research and development experience, but also has a large-scale production plant with a daily output of 2-3 tons. Whether it is product knowledge or production supply, we can provide professional and fast services and solutions. There are also many types of biological buffers currently produced, which can meet one-stop shopping. If you need it, please contact us for consultation and purchase!
3- (cyclohexylamine) -1-propanesulfonic acid, abbreviated as CAPS, is an important buffer and laboratory reagent. At present, the demand for it in the market is gradually increasing. In view of industry competition, various manufacturers are constantly adjusting prices to attract customer flow. So what is the connection between its price adjustment and? This article will answer everyone's doubts.