What are the uses of MES buffer and how to choose?
Release time:
2022-04-17
MES buffer is one of the earlier zwitterionic buffers described by Norman E. Good in 1966. Today it is commonly used in biological and biochemical research. By studying the properties of MES buffers and their applicability to different biotechnological applications (cell culture, chromatography, etc.), MES buffers are mainly used for the following 12 uses:
1. As an anion buffer, MES buffer is suitable for cation exchange chromatography and hydroxyapatite chromatography;
2. Can be used for gel filtration chromatography;
3. Can be used for phosphocellulose column chromatography;
4. Can be used for hydrophobic substance interaction chromatography;
5. Can be used for SDS-PAGE;
6. Can be used for fluorescence microscope;
7. Suitable as a non-coordinating buffer in metal ion solutions;
8. Because MES buffer does not form free radicals, it is suitable for the research of redox process;
9. Because it will not be metabolized by bacteria and eukaryotic cells, MES buffer can be used to prepare medium, such as low concentration plant cell medium, mammalian cell medium;
10. Suitable for most toxin studies;
11. For in vitro dissolution test of liposomes;
12.MES buffer interacts with the peptide backbone of bovine serum albumin to enhance protein stability.
So what should be considered before choosing an MES buffer for research?
1. It can be oxidized by H2O2, but since the oxidation occurs slowly, it is not expected to have a significant impact on biological/biochemical systems;
2. It can change the interaction of lipids;
3. When it is in its protonated form, it inhibits connexin channel activity in rat hepatocytes;
4. It interferes with phenolic oxidation through peroxidase;
5. High concentrations of MES buffer are toxic to most plants;
6. In high concentration MES buffer, the mobility of ions is low;
7. High concentration of MES buffer has lower conductivity;
8. Poor stability combined with most metal ions;
9. MES buffer is considered a good substitute for citrate, which can bind to certain proteins and form complexes with certain metals;
10.MES buffer is considered to be a good substitute for maleic acid which has a strong ability to absorb UV light.
Hubei Xindesheng Material Technology Co., Ltd. manufacturer of biological buffers, our products are shipped daily to top research centers and biotechnology companies in Europe, America and Asia. Our company can provide you with the following services:
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