Production and use of luminol luminescence reagent

Release time:


Luminol is the earliest chemiluminescence reagent used. This type of reagent was imported in the past, but now there are many domestic reagent manufacturers like Desheng that produce luminol. Here is a brief introduction to the production and use of luminol.

Luminol synthetic route

The chemical name of luminol is 3-aminophthalic hydrazide, CAS number: 521-31-3, and it can be prepared from 3-aminophthalic acid as a raw material. After dehydrating the raw materials, 3-nitrophthalic anhydride is generated. This intermediate can be used for organic synthesis and alcohol determination. Then through the nitration reaction, 3-nitro-phthalic acid can be obtained. At the same time, 4-nitro-phthalic acid will be obtained. The former product can be reacted with hydrazine (hydrazine) to obtain 3-aminophthalic hydrazide, which is luminol; the other product can be isoluminol.

Luminol Chemiluminescence

Luminol intermediate by-product separation

From the synthetic route of luminol, it can be known that there are by-products in its intermediates, which will generate isoluminol when reacting with hydrazine, so this by-product needs to be removed. In the 3-nitro-phthalic acid molecule, the nitro group has a great influence on the ortho carboxyl group. It will form an intramolecular hydrogen bond with the carboxylic acid, plus the intramolecular hydrogen bond between adjacent dicarboxyl groups. The dissociation of the entire carboxylic acid molecule produces a significant inhibitory effect, resulting in a decrease in its water solubility. In 4-nitro-phthalic acid, it is difficult to form intramolecular hydrogen bonds between the nitro group and the carboxylic acid. Therefore, its dissociation degree in water is relatively larger and the water solubility is better. The isomers produced after nitration of phthalic anhydride have differences in water solubility, which can be used to remove by-products.

Use of Luminol Luminescent Reagent

Luminol belongs to enzymatic chemiluminescence, and the detection needs to be catalyzed by peroxidase HRP, and then hydrogen peroxide can be added under alkaline conditions to stimulate the chemiluminescence reaction; while HRP can label proteins or antibodies, so it can Combined with immune response. Desheng has many years of experience in the research of chemiluminescence reagents. The luminescence reagents it can provide include luminol, isoluminol and a variety of acridinium esters.