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Before you buy Good's Buffer, you need to know about it.
Good's buffers are amphoteric ion buffers. These buffers are usually amino sulfonic acid organic compounds. When compounded into solutions, the pH value is relatively stable, and they do not react with a variety of enzymes and chemical reagents. They are mainly used in biology and medicine.
Good's buffers have the following commonalities
(1) High solubility in water;
(2) The acid-base dissociation constant (pKa value) is stable, generally at 6-8. The ability to release protons is stable, and is less affected by ion concentration, solution composition and temperature.
(3) It has low cell membrane permeability and is not easy to penetrate biofilm.
(4) It can chelate with low molar mass metal ions, but not precipitate with metal ions.
(5) High chemical stability;
(6) Light absorption is small in the wavelength range of ultraviolet and visible light.
(7) The possibility of salting out with organic polymers in organism is small.
(8) High purity salt is easy to obtain.
(9) Not participating in the biochemical reaction process, not interfering with the biochemical reaction process.
Usage Range of Common Good's Buffer PH
|
Chemical name |
Abbreviations |
CAS |
molecular weight |
pKa |
Use the PH range |
|
N,N-Bis(2-hydroxyethyl)glycine |
BICINE |
150-25-4 |
163.17 |
8.3 |
7.6 - 9.0 |
|
3-(Cyclohexylamino)-1-propanesulfonic acid |
CAPS |
1135-40-6 |
221.32 |
10.4 |
9.7-11.1 |
|
3-Morpholinopropanesulfonic acid |
MOPS |
1132-61-2 |
209.26 |
7.2 |
6.5 - 7.9 |
|
3-[[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]-1-propanesulfonic acid |
TAPS |
29915-38-6 |
243.28 |
8.55 |
7.7 - 9.1 |
|
4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid |
EPPS |
16052-06-5 |
252.33 |
8 |
7.3 - 8.7 |
|
PHOSPHOENOLPYRUVIC ACID TRIS(CYCLOHEXYLAMMONIUM) SALT |
PEP |
35556-70-8 |
465.56 |
|
|
|
2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid |
HEPES |
7365-45-9 |
238.3 |
7.5 |
7.0 - 7.6 |
|
Piperazine-1,4-bis(2-ethanesulfonic acid) |
PIPES |
5625-37-6 |
302.37 |
6.8 |
6.1 - 7.5 |
|
Tris(hydroxymethyl)aminoethane |
Tris |
77-86-1 |
121.14 |
8.1 |
7 - 9 |
|
Tris(hydroxymethyl)aminomethane Hydrochloride |
Tris-hcl |
1185-53-1 |
157.6 |
8.1 |
7.0 - 9.0 |
|
3-(N-Morpholino)-2-hydroxy-1-propanesulfonic acid |
MOPSO |
68399-77-9 |
225.26 |
6.75 |
6.2 - 7.6 |
|
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulphonic acid |
BES |
10191-18-1 |
213.25 |
7.1 |
6.4 - 7.8 |
|
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt |
BES-Na |
66992-27-6 |
235.23 |
7.1 |
6.4 - 7.8 |
|
2-(Cyclohexylamino)ethanesulfonic acid |
CHES |
103-47-9 |
207.29 |
9.3 |
8.6 - 10.0 |
|
3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid |
CAPSO |
73463-39-5 |
237.32 |
9.6 |
8.9 - 10.3 |
|
3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid |
DIPSO |
68399-80-4 |
243.28 |
7.6 |
7.0-8.2 |
|
N-(2-Acetamido)-2-Aminoethanesulfonic acid |
ACES |
7365-82-4 |
182.2 |
6.8 |
6.1 - 7.5 |
|
N-(2-acetamido)iminodiacetic acid |
ADA |
26239-55-4 |
190.15 |
6.6 |
6.0 - 7.2 |
Advantages that Desheng can provide good's buffer
BICINE：Buffer is recommended for low temperature biochemical work of biological buffer. It is used to prepare a stable substrate solution for the determination of guanine in serum. Buffer is also a widely used Buffer, used in enzyme reaction buffer, electrophoretic buffer, working concentration from 3 to 100 mM.
Especially for low-temperature biochemical research; used to prepare stable substrate solution for the determination of serum guanine enzyme; used cross-linked dextran to separate plasma proteins by isotachophoresis.
DESHENG BICINEI has an annual capacity of 200 tons.
CAPS：Biobuffer used in biochemical diagnostic kit: DNA/RNA extraction kit and PCR diagnostic kit: buffer used for enzymatic chemistry and HPLC separation of alkaline drugs. It is the raw material for manufacturing welding materials, air conditioning equipment and manufacturing. It can be used as analytical reagent, heat exchange carrier and pharmaceutical industry. For air conditioning, dry battery additives. For the paint industry, it is used as waterborne isohydrogenate curing agent.
DESHENG CAPS has an annual capacity of 350 tons.