Type of virus inactivated in sample preservation solution
The sample preservation solution can effectively and quickly inactivate the virus in the sample, improve the accuracy of nucleic acid detection results, and avoid the risk of virus spread during sampling, transportation, and detection. In fact, different preservation solutions also have different inactivation methods to meet a variety of related detection experiments.
Sample preservation solution lyses proteins to retain nucleic acids
This type of preservation solution is also a relatively widely used type, which is suitable for large-scale nucleic acid screening. It contains cleavage components that can cleavage the protein shell of the virus and destroy the secondary bond between the protein and RNA in the virus; the surfactant will also denature the protein and destroy the membrane structure of the virus to promote the cleavage process; some also add protease to the protein Cut into small fragments to further promote the separation of protein and nucleic acid, and quickly release nucleic acid. The preservation solution also contains biological buffers and Rnase inhibitors, which can protect the stability of the nucleic acid structure.
Usually the preservation solution only retains the nucleic acid of the virus. Every organism including the virus has a specific nucleic acid sequence. As long as the specific nucleic acid in the sample is detected, the diagnosis can be made, but it is not suitable for antigen detection; in addition, pseudoviruses are used. When performing verification experiments, because the nucleic acid is not the nucleic acid of the target virus to be tested, this type of sample preservation solution cannot be used.
Inactivate virus while preserving protein and nucleic acid
The essence of the sample preservation solution inactivating the virus is to make the virus lose its ability to infect, and sometimes it can retain its protein and nucleic acid at the same time. Inactivating the virus actually only needs to destroy the high-level structure of the virus protein, and the protein loses its physiological activity, and the virus loses its ability to infect, cause disease, and reproduce. It is not necessary to completely crack the virus protein. After the virus is inactivated by this sample preservation solution, it does not affect the primary structure of the protein, that is, the protein sequence does not change. In addition to identifying conformational epitopes (protein tertiary structure), antigen detection can also identify linear epitopes (primary structure). Therefore, this type of preservation solution can be used for nucleic acid detection after inactivating the virus, as well as antigen detection and protein detection.
In addition, the virus can be inactivated by heating at 80-100°C for 5-10 minutes without using the sample preservation solution. However, this method is not as simple and quick as the sample preservation solution inactivation. It is only suitable for laboratory testing and the amount of nucleic acid extraction. less. Desheng is a sample storage solution manufacturer, which can supply inactivated, non-inactivated, colorless, red and other types of storage solutions.
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.