Which biological buffers are suitable for electrophoresis gels?
Release time:
2021-04-14
The main components of electrophoresis cannot be separated from our biological buffer. They are usually added to the solution to stabilize the pH when current is passed through the sample. In addition, the buffer provides the ions required for electrophoretic migration. The ideal buffer for electrophoresis depends on the isoelectric point (pH at which a particular molecule has no net charge) of the sample being analyzed. Let's take a look at which buffers are suitable for electrophoresis gels, and what are their respective roles?
1. Tris buffer
Used for: gel electrophoresis (TAE or TBE solution)
Useful pH range: 7.5-9.0
pKa (25°C): 7.8-8.2
Molecular weight: 121.14
Function: Modify the gel structure and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis. The pH connection generated by the discontinuous buffer in capillary electrophoresis removes sample background buffer ions and myoglobin enrichment.
2. MOPS buffer
Used for: gel electrophoresis
Useful pH range: 6.5-7.9
pKa (25°C): 7.0-7.4
Molecular weight: 209.3
Function: Sepharose-formaldehyde gel electrophoresis to separate long RNA.
3. Bicine buffer
Used for: gel electrophoresis
Useful pH range: 7.6-9.0
pKa (25°C): 8.1-8.5
Molecular weight: 163.2
Function: A novel Bicine running buffer system, used to double the sodium lauryl sulfate-polyacrylamide gel electrophoresis of membrane proteins.
4. CAPS buffer
Used for: Capillary Electrophoresis
Useful pH range: 9.7-11.1
pKa (25°C): 10.2-10.6
Molecular weight: 221.32
Function: Use capillary electrophoresis-inductively coupled plasma sector field mass spectrometry to determine trace levels of element species. Capillary electrophoresis is used to detect and quantify alginic acid in mussels.
Whether it's Tris, MOPS, Bicine or CAPS buffers, we have the production of Desheng, and as a manufacturer, we will give you far more than the quantity of the products, as well as the quality of the products and excellent after-sales service.
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