Advantages of acridine ester chemiluminescence method for detecting interleukin 6
Chemiluminescence is a more advanced detection method in in vitro diagnosis, and it has gradually replaced enzyme-linked immunoassay as the mainstream detection method. Commonly used chemiluminescence reagents, acridinium esters, are widely used, and are very suitable for the labeling of various macromolecules such as proteins, antibodies, DNA, RNA, etc., such as the detection of interleukin 6 by the acridinium ester chemiluminescence method.
Interleukin-6, or interleukin-6 (IL-6), is a multi-effect cytokine, a kind of interleukin, composed of fibroblasts, monocytes/macrophages, T lymphocytes, and B lymphocytes. , Epithelial cells, keratinocytes and a variety of tumor cells. Its main functions are: to promote the proliferation and differentiation of B cells and to secrete antibodies, and it also has a wide range of effects on hepatocytes, T cells, neural tissues, and hematopoietic system; it has anti-tumor effect and can directly or indirectly enhance the tumor-killing activity of NK cells and CTL . It has many synonyms, such as beta 2 interferon, hepatocyte stimulating factor, hybridoma growth factor, B cell differentiation factor and so on.
Acridine ester chemiluminescence detection
Interleukin 6 plays an important role in the body's defense mechanism, and also plays an important role in the occurrence of many diseases. Its detection is very important for understanding the disease, judging the prognosis, and studying the pathogenesis.
Advantages of acridine ester chemiluminescence method for detecting interleukin 6:
In the past, interleukin 6 detection kits mostly used enzyme-linked immunosorbent assay. The disadvantage of using enzyme-linked immunoassay for detection is that horseradish peroxidase or alkaline phosphate markers are easy to inactivate, the chromogenic substrate is easy to decompose when exposed to light, and the sensitivity is low. It has a certain cross-reactivity to compounds with similar structures, causing the test The result is not accurate. However, the current sampling of acridinium ester chemiluminescence method to detect interleukin 6 has the following advantages:
① The reaction does not require a catalyst, the background luminescence is low, the signal-to-noise ratio is high, and the interference factors of the luminescence reaction are few;
② Flash type luminescence, rapid and concentrated light release, high luminous efficiency, and high luminous intensity;
③The molecular weight of acridinium ester is small, which avoids shielding antibody binding sites and improves the overall sensitivity of the system;
④ It is easy to connect with protein and the photon yield does not decrease after the connection; ⑤The label is stable, not affected by environmental oxidants, and can be stored for several months at 2-8°C. Therefore, acridine substitution is a very effective chemiluminescent label.
In addition to labeling and detecting interleukin 6, the acridinium ester chemiluminescence method can also be used for the labeling and detection of new crown antibodies. It is a highly sensitive and highly automated antibody detection method. Desheng has developed six different acridinium ester or acridine sulfonamide reagents based on the difference of labeled proteins, antibodies, nucleic acids and other macromolecules.
When it comes to the chemiluminescent reagent Luminol, it is not unfamiliar to everyone and is commonly used in the field of criminal investigation to detect blood stains. Researchers have found that the principle of luminol luminescence can be used to detect specific substances in saliva, such as proteins, DNA, and related indicators of microorganisms, in order to determine physical conditions.