Why does high blood anticoagulant concentration cause hemolysis?
When we configure the anticoagulant in blood vessel harvesting, we sometimes encounter such problems as hot weather and fast blood coagulation. If the anticoagulant EDTA dipotassium is not effective, we need to increase the amount of EDTA dipotassium. Then we use the reagent with the original configuration concentration to add a little more or to add the same amount. How about increasing the concentration of the sample?
Sometimes when we increase the concentration, we will encounter local hemolysis after blood collection, which will have a great impact on blood testing. This is actually the reason why the concentration of anticoagulant is high and the osmotic pressure is high.
Speaking of osmotic pressure, people who may not understand it are not easy to understand, and a daily example is very easy to understand. We all know that when we cut a wound on our hand, especially when it's deep, we can't rinse it with clean water. In fact, this is also related to osmotic pressure. Our wound cells do not have skin protection. When contacted directly with clean water, the osmotic pressure in cells is high, while clear water does not have osmotic pressure. Water will permeate from one side of low osmotic pressure to the other side of high osmotic pressure, and the cells will continuously absorb water. Our human body's animal cells are unlike. Plant cells have cell walls. When they absorb too much water, the cell membranes will burst quickly, and then the cells will die, even the wounds will fester. Therefore, medical alcohol should be used to clean the wound, but not water. The osmotic pressure of medical alcohol is equal to that of cell liquid, and it will not cause rupture. In addition, the concentration of saline and injected glucose is also required, which is also related to osmotic pressure.
Similarly, when we increase the concentration of anticoagulant, although the amount added to the blood collection is very small, the concentration will be much lower after blood dilution, it seems that there will be no osmotic pressure problem. But in fact, the blood is slowly pumped into the blood collection. When the blood is just in contact with the anticoagulant on the wall of the blood collection vessel, the anticoagulant has not been able to mix and dilute with the blood uniformly. Local cells will dehydrate because of the high external osmotic pressure. Our red blood cells are very fragile, and the cell membrane will rupture, thus causing the situation. Hemolysis of the part.
Therefore, when we need to increase the dosage of anticoagulant, we should give priority to increasing the dosage of anticoagulant. It is better not to increase the concentration of anticoagulant. Under the same conditions, the concentration is proportional to osmotic pressure, and the higher the concentration, the higher the osmotic pressure; as long as the dosage is not too much, the effect can be ignored.
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