The role of glucose dehydrogenase in different fields
Glucose dehydrogenase (EC 18.104.22.168) is an oxidoreductase that can be obtained from a variety of microorganisms and tissues. It is a white amorphous powder, and α-galactosidase catalyzes the hydrolysis of α-galactosidic bonds, which can transform and decompose the anti-nutritional factor α-galactosides in feed and soybean foods, and improve its nutritional content. In addition, the enzyme also has certain applications in pharmacy, thickener treatment and paper industry.
Mechanism of action
Glucose dehydrogenase specifically oxidizes and decomposes glucose molecules to produce gluconic acid through the pentose phosphate pathway, accompanied by the production of hydride ions. The function of this enzyme also requires the participation of oxidized coenzyme NAD+ or NADP+. The electron acceptor NAD+ or NADP+ quickly combines with the generated hydride ions to generate reduced coenzyme NADH or NADPH, forming a cofactor regeneration cycle.
Glucose dehydrogenase is used to oxidize and decompose glucose molecules to generate gluconic acid; interact with oxidized coenzyme NAD+ or NADP+ to generate reduced coenzyme NADH or NADPH; work with ketoreductase and coenzyme to produce specific carbonyl compounds with high optical purity The chiral alcohol.
Glucose dehydrogenase can also be widely used in food industry and pharmaceutical industry. It is reported that the yield of D-ribose produced by the glucose dehydrogenase method is positively correlated with the activity of GDH. D-ribose, or D-ribofuranose, is a constituent of ribonucleic acid and nucleotide derivatives of many coenzymes in organisms, and has very important physiological functions. In the biosynthetic pathway, GDH is its rate-limiting enzyme. As an oxidoreductase, GDH can be made into a biosensor. In addition, GDH can be used as a diagnostic enzyme for clinical blood glucose measurement. The GDH enzymatic method is used to measure blood glucose. Compared with the traditional methods, glucose oxidase (GOD) method and hexokinase (HK) method, the operation is simple, the cost is low, the one-step method, a single enzyme can be completed, and the continuous detection method or the end point method is used for analysis. , Can be used in several automated analyzers. This method is not interfered by anticoagulants or preservatives in general concentrations and has high sensitivity. At present, the glucose dehydrogenase method is considered by most people as a reference method for blood glucose determination.
Trihydroxymethylaminomethane is an important laboratory reagent widely used in fields such as molecular biology and biochemistry. To ensure its quality and purity, a series of tests are required. This article provides measurement methods for appearance, content, solubility, drying weight loss, pH value, melting point, and UV absorbance. These methods help to evaluate the quality and purity of trimethylaminomethane.