Does lithium heparin and sodium fluoride anticoagulation affect the detection of HbAlc?
As we all know, glycosylated hemoglobin (HbAlc) is the first choice for evaluating diabetes blood glucose control level. In recent years, capillary electrophoresis has been introduced for the first time as a new HbAlc detection method. Some studies have shown that this method has anti-interference ability against common abnormal hemoglobin. It is better and can indicate thalassemia, and has better performance, and is gradually being used in clinical practice. However, there is no report on the factors that influence the different types of anticoagulation, storage conditions and other aspects of the whole blood specimens used in this method. Therefore, in this experiment, samples treated with conventional doses of lithium heparin and sodium fluoride anticoagulant were used to preliminarily explore whether the method has an effect on the detection of HbA1c, in order to evaluate the applicability of lithium heparin and sodium fluoride anticoagulant samples.
The detection principle of capillary electrophoresis is to separate charged molecules in an alkaline buffer with a specific pH value according to their electrophoretic mobility. This method has high resolution. By using alkaline pH buffer, normal and abnormal (Or variants) hemoglobin is detected in the following order, from cathode to anode: A2/C, E, S/D, F, A0, other Hb (including minor HbA1) and A1c, which are unique to hemoglobin Under the absorption wavelength of 415nm, it can be directly and accurately detected at the cathode end of the capillary, so as to perform relative quantitative analysis of the HbA1C component.
The study found that compared with the anticoagulant EDTA-K2 anticoagulant specimens recommended by the manufacturer, when using conventional doses of lithium heparin and sodium fluoride for anticoagulation, the results of capillary electrophoresis detection of HbAlc and EDTA-K2 anticoagulation specimens showed no difference It was statistically significant (P>0.05), and was correlated with the test results of EDTA-K2 anticoagulant specimens (P<0.001), indicating that lithium heparin and sodium fluoride did not interfere with the detection of HbAlc by capillary electrophoresis. Two kinds of anticoagulant anticoagulant specimens are suitable for the detection of HbA1c by capillary electrophoresis.
In daily work, lithium heparin and sodium fluoride are the main daily anticoagulants used in the anticoagulation of clinical biochemical project test specimens. This study found that lithium heparin and sodium fluoride anticoagulation are also suitable for the detection of HbAlc by capillary electrophoresis. For diabetic patients who need to check the clinical biochemical items and HbAlc concentration at the same time, it can avoid more blood sampling, especially for elderly patients, it is easy to accept and convenient for blood sampling. This also shows that the capillary electrophoresis method for detecting HbAle is more convenient for clinical application than traditional methods and has advantages.
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