Luminescent materials combined with bioimaging technology help early diagnosis of liver injury

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Liver diseases such as hepatitis, liver cirrhosis, fatty liver, and acute liver failure are extremely harmful, and early diagnosis is beneficial to the prevention and treatment of such diseases. In common liver function test items, in vitro diagnostic reagent materials play an important role. In addition, molecular fluorescent probes are also indispensable research methods in modern life sciences and disease diagnosis. Recently, researchers from the Army Military Medical University and the People’s Hospital of Wuhan University have jointly developed a biodegradable luminescent material, and combined with fluorescence imaging technology to prepare a nanoprobe to achieve neutrophil-mediated acute Luminescence imaging of liver injury is of great significance for the diagnosis, prevention and treatment of liver injury.

Fluorescent dyes are widely used in various fields such as biomolecular labeling, enzyme analysis, environmental monitoring, cell staining and clinical laboratory diagnosis, and molecular fluorescent probes based on organic fluorescent dyes have high sensitivity, simple operation, good reproducibility, Many advantages such as good membrane permeability and in-situ detection are convenient for in-situ real-time non-damage detection of target molecules in biological systems, and can be used to monitor living cells and biological molecules and their biological processes in living bodies. In this study, the researchers developed biodegradable luminescent materials through chemical functionalization of cyclic oligosaccharides, and made a kind of nanoprobes (LaCD NPs) based on this.

The luminescence of the nanoprobe depends on the level of reactive oxygen species and myeloperoxidase (MPO). Correspondingly, activated neutrophils can be specifically imaged by LaCD NP, and the luminescence signal is positively correlated with the neutrophil count. In mouse models of alcoholic liver injury (ALI) and acute liver failure (ALF), LaCD NP can accurately quantify and track neutrophils in the liver. In both cases, the changes in probe luminescence intensity are consistent with the changes over time in neutrophils, MPO and other parameters related to the pathogenesis of liver injury. In addition, surface functionalization using neutrophil targeting peptides can further improve the luminescence imaging capabilities of LaCD NPs.

Moreover, the researchers verified the good safety of LaCD NP through preliminary in vitro and in vivo studies, which can be further developed into an effective and biocompatible luminescent nanoprobe, which can not only be used for dynamic detection of neutrophil-mediated in vivo The occurrence of acute liver injury (ALI and ALF) is also expected to be used to diagnose other neutrophil-related liver diseases.

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