Desheng talks about the problems encountered in the development of China's enzyme preparation industry
Industrialized enzyme preparations are called enzyme preparations, which are biological products with catalytic function after enzymes have been purified and processed, and are mainly used to catalyze various chemical reactions in the production process. It has the characteristics of high catalytic efficiency, high specificity, mild operating conditions, reduced energy consumption, and reduced chemical pollution. It is widely used in food, textile, feed, lotion, paper, leather, medicine, energy development, environmental protection, etc. . Today we mainly talk about enzyme preparations used as raw materials for diagnostic kits. Desheng is one of the manufacturers of enzyme preparations, and the enzyme preparation products provided are mainly used in kit detection.
The development process of the enzyme preparation industry:
The earliest enzyme preparation industry in China originated in the middle of the last century, and the product was mainly BF7658α-amylase used in textile desizing. In the late 1990s, high-temperature α-amylase and high-conversion liquid glucoamylase began to be widely used in alcohol, monosodium glutamate, sugar, beer and other industries. At that time, Chinese enzyme preparations were mainly amylase and glucoamylase, and the total amount of both accounted for more than 80% of total exports. Now China's enzyme preparation products have been updated, and now there are more and more industrial enzymes, and more manufacturers are importing enzyme preparations from abroad. The fundamental reason is that there are too few domestic enzyme preparation manufacturers and the product quality varies.
Problems in the enzyme preparation industry:
After decades of development, China's enzyme preparation industry grew out of nothing and initially formed a certain scale. In the development process, due to the late start of China's enzyme preparation industry, it is under competitive pressure from foreign companies in the same industry. In recent years, China's enzyme preparation industry has developed rapidly and has made significant progress. However, from the perspective of the world's enzyme preparation industry, the application of high technology in the world's enzyme preparation industry has further widened the gap between China's enzyme preparation industry and the world's advanced level. The entire industry has a small production scale, weak technology research and development capabilities, relatively single products, and irrational structure.
1. Insufficient R&D investment and R&D innovation capabilities. Chinese enzyme preparation companies spend less than 4.5% of their product sales on average for developing new products and improving production processes, and their personnel input is also seriously insufficient. The main reason is that domestic enzyme preparation companies are relatively small, and the total annual R&D investment of enterprises in the industry is less than 100 million yuan. However, since the enzyme preparation industry is a technology-intensive industry, a large amount of capital investment is required. The serious shortage of R&D investment has led to weak technical reserves of Chinese enzyme preparation companies and faced huge challenges in development.
2. The level of production technology and equipment is relatively backward. The production technology level of China's enzyme preparation industry is lagging behind that of foreign countries. The production of domestic enzyme preparations mostly uses more traditional fermentation, separation and extraction techniques and preparation preparation techniques, causing serious waste of resources, low product purity, and low product quality. In the production process of foreign enzyme preparations, technologies such as genetic engineering, protein engineering, artificial synthesis, simulation and directed evolution are widely used. Among them, high-tech achievements based on genetic engineering and protein engineering have been industrialized in the field of enzyme preparations, bringing revolutionary development to the enzyme preparation industry. Domestic research in this field is still relatively weak, and the application technology of independently innovative enzyme preparations is backward, which has become the main limiting factor for the further expansion of China's domestic enzyme preparation market.
3. Unreasonable product structure and insufficient application depth of enzyme preparations. Chinese enzyme preparations are still dominated by traditional enzyme preparations (glucoamylase, amylase, protease, etc.), lacking high-end enzyme preparation products, and the structure is unreasonable. At the same time, due to the existence of single varieties and vicious competition, the economic benefits of China's enzyme preparation industry are poor. At present, foreign enzyme preparation companies are vigorously developing liquid formulations and granular products, focusing on the development of immobilized enzymes. Due to the relatively backward extraction technology, technology and equipment level of domestic enzyme preparations, the products are mainly crude enzymes, the quality is uneven, and the application is restricted. The mainstream of international enzyme preparations is compound enzymes with high added value. The development of compound enzymes needs to be based on the comprehensive technology of enzyme preparation production and application. Domestic enzyme preparation companies lack application workshops and professional researchers in the production and application of enzyme preparations, and lack the conditions for proposing corresponding solutions based on raw materials, processes and equipment conditions, which restricts the further rapid development of China's enzyme preparation industry.
In chemiluminescence analysis, the luminescence intensity of acridine ester is influenced by various factors, such as reaction medium, temperature, time, and excitation light source energy. To achieve good detection results, it is necessary to comprehensively consider and optimize these factors. Meanwhile, attention should be paid to controlling and standardizing experimental conditions to ensure accurate and reliable results. Thoroughly studying these influencing factors will help promote the development of chemiluminescence analysis methods.