In the complex process of clinical testing, coagulants are often considered as potential factors that affect the test results. When abnormal triglyceride detection occurs, clinical workers often ask: Does the coagulant interfere with the accuracy of the detection? In fact, scientific analysis shows that there is no direct correlation between coagulants and abnormal triglycerides. The true factors affecting quality are often hidden in various stages of specimen processing. Understanding the correct mechanism of action of coagulants is the first step in improving the quality of testing.

blood coagulants

The mechanism of action of coagulants

The main function of coagulants in blood testing is to accelerate the coagulation process and assist in rapid separation of serum. The common coagulant is inorganic silicon-based material, which physically activates the endogenous coagulation pathway and promotes fibrin formation. This process is completely independent of the lipid metabolism pathway and does not undergo any chemical reaction with triglycerides. From the perspective of its mechanism of action, coagulants target the coagulation cascade reaction, while triglyceride detection is based on specific enzymatic reactions. The two systems are completely different in their biochemical pathways, and there is no possibility of cross interference. Standardized use of coagulants will not alter the molecular structure or detection characteristics of triglycerides.

Standardization requirements for centrifugal operation

The operating procedures of centrifugation directly affect the quality of serum, and improper centrifugation conditions may lead to the mixing of lipoprotein particles into the serum. Insufficient centrifugal force or insufficient centrifugation time can prevent the complete precipitation of large molecular lipoproteins such as chylomicrons and very low-density lipoproteins, which can directly interfere with the detection results of triglycerides if these particles remain in the serum. The standardized centrifugation operation requires centrifugation at a relative centrifugal force of 2000g for 10-15 minutes to ensure complete separation of serum and blood cell components.

Quality assurance of blood collection tube cleanliness

The cleanliness of blood collection tubes and additives is also an important factor affecting the test results. If the container is not thoroughly cleaned during the process of adding coagulants, exogenous impurities may be introduced, which may generate interference signals during the detection process. Especially some lipid soluble pollutants may directly interfere with the enzymatic detection of triglycerides. Therefore, strict control of cleaning conditions is required throughout the entire process from production to use.

Quality control of coagulants: ensuring reliability from the source

The quality of the coagulant itself is also an important factor affecting the detection. High quality coagulants should have stable chemical composition, consistent particle size, and good dispersibility. These characteristics ensure that the coagulant can function uniformly and effectively. Selecting reliable coagulant suppliers and establishing a comprehensive acceptance and usage evaluation system can control the quality of testing from the source. Regularly verifying the performance of coagulants to ensure they meet testing requirements is an important aspect of laboratory quality management.

Product packaging

In summary, there is no direct correlation between coagulants and abnormal triglycerides. The key to ensuring the accuracy of triglyceride detection results lies in strictly controlling the standardization of centrifugation operations, the cleanliness of blood collection tubes and additives, and the quality control of coagulants themselves. Hubei Xindesheng is a high-tech enterprise specializing in the research and production of diagnostic reagents. The company has profound technical accumulation and rich practical experience in the field of blood collection tube additives, especially in the research and production of blood coagulants. It has achieved very good results. If you need it, please feel free to visit the factory.