In the world of biochemistry, buffer solutions are like "guardians" that maintain reaction equilibrium. Among them, N, N-dihydroxyethylglycine (Bicine buffer) has shown unique value in cutting-edge fields such as protein electrophoresis and enzyme kinetics research due to its excellent buffering performance. To maintain the excellent performance of this buffering reagent, it is necessary to master the secrets of its preservation, and factors such as temperature control are the key to success or failure.

Bicine buffer

Temperature: A Line of Defense for Stability Protection

As a fine organic compound, Bicine is particularly sensitive to temperature changes. Research has shown that storing it at room temperature between 4 ° C and 25 ° C is most ideal. This temperature range can ensure the integrity of the molecular structure and avoid the risk of decomposition caused by temperature fluctuations. If the temperature is too high, the refrigeration scheme needs to be activated, and controlling the temperature between 2 ° C and 8 ° C can prolong its stability. However, it should be noted that when storing under refrigeration, the container should be completely sealed to prevent concentration changes caused by moisture condensation.

Environmental factors: potential threats that cannot be ignored

In addition to temperature as the main factor, the lighting and humidity in the environment also need to be strictly guarded against. The active groups in Bicine molecules are prone to photodissociation under strong light irradiation. Therefore, it is necessary to choose brown glass bottles for packaging or storage in a dark cabinet. At the same time, humid environments can cause problems such as reagent agglomeration and hydrolysis, especially during the southern rainy season, and moisture-proof measures cannot be ignored. It is recommended to place silica gel desiccant in the storage container and ensure that it is sealed immediately after each use.

Daily management: Details determine the success or failure of experiments

In practical laboratory operations, seemingly small details often determine the final effect of reagents. When using Bicine powder, be sure to keep the spoon dry and clean to avoid introducing impurities; Fresh ultrapure water should be used when preparing the solution to prevent ion interference; For prepared buffer solutions, it is recommended to label the preparation date and expiration date, and follow the principle of "first in, first out" for use. It is particularly important to note that Bicine solution taken out of the refrigeration environment should be allowed to stand at room temperature before use, and should only be used after reaching equilibrium with room temperature.

Long term preservation: a systematic solution

For laboratories that require large-scale or long-term preservation, establishing a complete preservation system is crucial. This includes: establishing a dedicated buffer storage area and equipping it with continuous temperature monitoring equipment; Develop standard operating procedures to standardize every step from opening, retrieval to re storage; Establish reagent files to record the usage and status changes of each batch of products. Through systematic management, not only can Bicine ensure long-term stability, but it can also effectively improve laboratory work efficiency and data reliability.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. has always been committed to providing users with high-purity and highly stable biological buffer products. The Bicine produced and developed by it is provided in the form of high-purity powder, which is not only convenient for long-distance transportation and long-term storage, but also enables ready to use, reducing activity loss and external interference during storage. Choosing New Desheng means choosing to control the quality of experiments from the source. If you are looking for stable and reliable Bicine raw materials, please visit the official website of Hubei Xindesheng and click on the online customer service consultation to obtain product details, technical parameters and other information, providing solid support for your scientific research journey!