Guidelines for the use of chromogenic substrate ADOS: Standardized operation to ensure the reliability of experimental results
Release time:
2025-12-05
In biochemical testing, the performance of chromogenic substrates directly affects the accuracy and repeatability of the test results. ADOS (CAS number: 82692-96-4) is a commonly used chromogenic substrate in peroxidase systems, which can undergo oxidation reaction with 4-aminoantipyrine (4-AAP) in the presence of hydrogen peroxide to produce colored products. This reaction has the characteristics of fast response and stable signal, and is suitable for various in vitro diagnostic kits and laboratory testing projects. This article introduces the basic properties, configuration methods, and storage suggestions of ADOS based on practical usage scenarios, to help users standardize the operation process.
1、 Basic characteristics and reaction principles of ADOS
The chemical name of ADOS is N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline sodium salt, which is a water-soluble derivative of aniline. Under the catalysis of peroxidase (such as horseradish peroxidase HRP), it can react with 4-AAP and hydrogen peroxide to generate purple red quinone imine compounds. This reaction has been widely used in colorimetric determination of glucose, cholesterol, uric acid and other items.
2、 Product Appearance and Packaging Instructions
ADOS is typically provided in the form of white crystalline powder, packaged in dark brown glass bottles to reduce the impact of light on reagent stability. The product is transported in foam incubator, containing ice bag, and attached with instructions and quality inspection report. If the ice pack has melted when receiving the goods, it generally does not affect the product quality because ADOS has a certain stability at room temperature. Low temperature transportation is mainly to cope with the high temperature environment that may occur during transportation. However, it should be noted that if the powder color noticeably turns yellow, gray, or clumps, it may have undergone oxidation or been contaminated by microorganisms. It is recommended to stop using it.
3、 Precautions for Solution Preparation
ADOS can be directly dissolved in deionized water or ultrapure water, with good solubility. Before opening the bottle, low-speed centrifugation can be performed to recover powder that may adhere to the bottle cap or wall, reducing losses. If the dissolution rate is slow, a 37 ℃ water bath or short-term ultrasound assistance can be used appropriately to avoid prolonged heating or vigorous stirring. The prepared solution should be used as soon as possible and should not be stored for a long time, as it is easily oxidized by oxygen in the air in aqueous solution, resulting in a decrease in color development efficiency. If the experimental system must use organic solvents such as DMSO, the solubility of ADOS in it should be confirmed first, and a corresponding concentration of solvent control group should be set up to eliminate interference.
4、 Storage conditions and expiration date management
Unopened ADOS powder is recommended to be stored at low temperature, away from light, and frozen for a longer period of time to maintain stability; Short term use can be placed in a dry environment at 4 ℃. Once opened, it should be sealed and stored as soon as possible, and the reagent status should be checked regularly to avoid experimental deviations caused by reagent deterioration.
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