In the field of in vitro diagnostics, chemiluminescence immunoassay technology holds an important position due to its high sensitivity and wide linear range. As a key component in chemiluminescence reagents, the luminescence efficiency of acridine ester directly affects the performance of diagnostic kits. As an important supplier of acridine esters, Hubei Xindesheng's production of acridine esters has attracted much attention in terms of luminescence efficiency.

Acridine ester powder

The luminescence mechanism basis of acridine ester

Acridine ester molecules have an acridine ring structure, which is the core basis for their luminescence. Taking the common acridine ester models produced by Xindesheng, such as acridine ester NSP-SA-NHS, as an example, the acridine ring in its molecule can undergo specific reactions with hydrogen peroxide ions in alkaline hydrogen peroxide solution. When hydrogen peroxide ions attack acridine ester molecules, the substituent on the acridine ring forms an unstable ethylene oxide intermediate with C-9 on the acridine ring and hydrogen peroxide. This intermediate is extremely unstable and rapidly decomposes into carbon dioxide and N-methylacridone in an electronically excited state. When N-methylacridone returns from the excited state to the ground state, it releases excess energy in the form of photons with an emission wavelength of approximately 430 nm, resulting in a detectable chemiluminescence signal. Unlike some other chemiluminescence reagents that require complex catalytic systems, acridine esters use direct chemiluminescence mode. This direct luminescence mechanism simplifies the reaction system and reduces background interference caused by other factors such as catalysts.

Internal factors affecting the luminescence efficiency of Xin Desheng acridine ester

（1） Structural modification enhances stability and luminescence performance

New Desheng has carried out a series of optimized modifications to the structure of acridine esters. Traditional acridine esters may have stability issues such as hydrolysis in certain environments, which can affect their luminescence efficiency. New Desheng has enhanced the hydrolysis resistance of acridine esters by increasing steric hindrance and other methods. This enhanced stability helps to maintain the structural integrity of acridine esters during storage and use of diagnostic kits, thereby maintaining luminescence.

（2） High purity raw materials ensure consistent luminescence efficiency

In the production process, Xindesheng strictly controls the quality of raw materials and uses high-purity raw materials for the synthesis of acridine esters. High purity raw materials reduce the interference of impurities on the reaction, ensuring that each batch of acridine esters produced maintains a high degree of consistency in structure and performance. In the production of diagnostic kits, consistent acridine ester products can ensure stable luminescence efficiency of different batches of kits, avoid fluctuations in test results caused by differences in reagents, and provide reliable testing basis for clinical diagnosis.

Actual luminous efficiency performance of Xindesheng acridine ester in diagnostic reagent kit

（1） High sensitivity detection reflects luminescence

In terms of infectious disease pathogen antibody detection, such as hepatitis B surface antibody, hepatitis C antibody, etc., Xindesheng acridine ester can sensitively detect micro antibodies in the blood by virtue of its fast luminous efficiency. Even with extremely low antibody levels, the strong luminescent signal generated by acridine ester can be captured by detection equipment, greatly improving the accuracy and timeliness of infectious disease diagnosis.

（2） Rapid detection benefits from rapid luminescence

The chemiluminescence reaction speed of acridine ester is extremely fast, and Xindesheng's acridine ester products also have this advantage. In the diagnostic kit, it only takes about 0.4 seconds from adding the luminescent starter reagent to reaching a high emission intensity, and the entire luminescent process can be completed within a few seconds. This fast luminescent characteristic greatly shortens the detection process and improves detection efficiency, especially suitable for clinical scenarios that require rapid acquisition of detection results, such as emergency testing.

Product packaging

The acridine ester produced by Hubei Xindesheng exhibits excellent luminescence efficiency in diagnostic kits due to its luminescence mechanism, rational structural design, and strict control over production materials and quality. By controlling internal and external influencing factors, its acridine ester products can stably exhibit their luminescent properties in different application scenarios, providing assistance in improving the performance of in vitro diagnostic kits. If you have any purchasing needs, please feel free to contact us at any time!