1、 Blood lipid health: the focus of modern medicine's attention

With changes in people's lifestyles, cardiovascular disease has become the "number one killer" threatening human health. Among numerous risk factors, abnormal blood lipids, especially abnormally elevated cholesterol levels, are important triggers for various cardiovascular diseases. Therefore, accurate and rapid detection of total cholesterol (TC) is particularly important. Behind this test, there is a seemingly small but crucial 'behind the scenes hero' - cholesterol oxidase.

cholesterol oxidase

2、 Principle of Total Cholesterol Detection: Enzymatic Reaction Process

At present, the total cholesterol detection methods widely used in clinical laboratories are mostly based on enzymatic determination, with the core being the conversion of cholesterol into detectable products through a series of enzymatic reactions. This process usually includes two key steps: first, cholesterol esters are hydrolyzed into free cholesterol under the action of cholesterol esterases; Subsequently, free cholesterol reacts with oxygen under the catalysis of cholesterol oxidase to produce cholestenone and hydrogen peroxide. The generated hydrogen peroxide undergoes a color reaction with a color reagent through peroxidase, and finally the absorbance is measured by a spectrophotometer to calculate the concentration of total cholesterol.

3、 High purity enzyme preparation: the core element to ensure accurate detection

The activity, purity, and stability of enzymes directly determine the detection sensitivity, linear range, and anti-interference ability of the reagent kit. If the purity of cholesterol oxidase is insufficient, non-specific reactions may be introduced, leading to false positive or false negative results; If the enzyme activity is insufficient, the reaction rate will be slow, affecting the detection efficiency; If the stability is poor, the validity period of the reagent kit will be greatly reduced, increasing the cost of clinical use. Therefore, companies developing and producing total cholesterol test kits should choose cholesterol oxidase with high specific activity, high specificity, and good batch stability as the core raw material.

4、 Promoting the upgrading of in vitro diagnostic industry: the path of localization of enzyme preparations

For a long time, the enzyme preparation market has been monopolized by foreign companies, resulting in high costs and the risk of unstable supply chains. In recent years, with the rapid development of biotechnology in China, more and more local enterprises have begun to make efforts in the field of enzyme preparations, committed to achieving independent and controllable control of key raw materials. In this context, domestic enzyme production enterprises with independent intellectual property rights and mastery of core fermentation and purification technologies are becoming the backbone of promoting the high-quality development of China's in vitro diagnostic industry.

5、 Hubei Xindesheng: Focusing on enzyme preparation research and development

Among numerous innovative enterprises dedicated to the localization of enzyme preparations, Hubei Xindesheng stands out with its profound technical accumulation and strict quality management system. As a professional enzyme manufacturer, Desheng New Materials focuses on the research and production of various diagnostic enzymes such as cholesterol oxidase, cholesterol esterase, peroxidase, etc. The company adopts advanced microbial fermentation technology and chromatographic purification technology to ensure that each batch of cholesterol oxidase has high activity, high purity, and reliable stability.