Lighting up the Light of Science: Unveiling the Production and Application of Luminol Luminescent Reagents
Release time:
2025-08-28
Luminol, also known as 3-amino-phthalimide (CAS number: 521-31-3), is a historically significant chemiluminescent reagent. It is used for forensic identification of blood traces and is known for its ability to react with bloodstains in the dark and emit blue light. With the development of time, the application scope of luminol has gradually expanded to multiple fields such as biomedical, environmental monitoring, and food safety, becoming an important tool in the hands of researchers. Here will be a brief introduction to the production and usage methods of luminol.
luminol powder
Luminol synthesis route revealed
The synthesis of luminol is a multi-step process involving various organic chemical reactions. Firstly, starting from 3-aminophthalic acid, dehydration reaction is carried out under specific conditions to generate 3-nitrophthalic anhydride, a key intermediate. Next, 3-nitro-phthalic acid is obtained through nitration reaction, which reacts with hydrazine to produce luminol. Luminol stands out among many applications due to its high luminescence intensity and low background signal.
Application scenarios of luminol
The working principle of luminol luminescent reagent is based on enzymatic chemiluminescence reaction. Specifically, when peroxidase (HRP) is present, luminol can produce blue light in alkaline environments. This characteristic makes luminol highly suitable as a marker in immunoassays. For example, in ELISA (enzyme-linked immunosorbent assay), HRP labeled antibodies or antigens can specifically bind to target molecules, and then add luminol and hydrogen peroxide to quantitatively analyze the concentration of the analyte in the sample by measuring the generated light intensity. In addition, luminol is widely used in fields such as DNA sequencing and cell imaging, helping scientists uncover the mysteries of life sciences.
The Rise of Domestic Luminol
In recent years, with the continuous investment of domestic enterprises in the development of luminol and related products, a number of domestic brands have emerged, and Desheng is one of them. Desheng has been dedicated to the research of chemiluminescence reagents for many years, accumulating rich experience and professional knowledge. The company provides a wide variety of luminescent reagents, including but not limited to luminol, isoluminol, and various acridine ester compounds. Through independent research and technological innovation, enterprises such as Desheng have promoted the progress of chemiluminescence technology in China, providing a solid material foundation for more scientific research.
Product packaging
Luminol luminescent reagent, as a bridge connecting theory and practice, has witnessed every step of scientific and technological development. From early experiments to today's automated detection systems, luminol has always played an indispensable role. In the future, with the continuous emergence of new materials and technologies, we have reason to believe that luminol and its derivatives will continue to shine on a broader stage, adding new brilliance to the boundaries of human knowledge.
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