Luminol: ushering in a new era of protein imprinting
Release time:
2025-08-25
Western blotting is an indispensable technique in modern biochemistry and molecular biology research. This technology achieves the detection, quantification, and analysis of specific proteins in cell or tissue samples through the specific binding of specific antibodies to target proteins. With the advancement of technology, the application of luminol as a luminescent substrate in protein blotting is becoming increasingly widespread, bringing new breakthroughs to scientific research.
Luminol - A More Sensitive and Safer Choice
Traditional protein imprinting methods, such as enzyme labeled substrate colorimetric or isotope labeled autoradiography, have their own advantages, but they have some limitations. For example, enzyme labeled substrate colorimetric method may require a longer time to obtain results, while autoradiography method involves the use of radioactive substances, which not only increases the safety risk of the experiment but also makes waste disposal more complex. In contrast, luminol, as a chemiluminescence reagent, can provide highly sensitive detection in a short period of time and avoid the use of radioactive substances, making it safer and more environmentally friendly.
The working principle of luminol
Luminol can be catalyzed by peroxidase (HRP) in the presence of hydrogen peroxide, which releases photons and produces visible light. When the protein sample is separated by polyacrylamide gel electrophoresis (PAGE) and transferred to the nitrocellulose membrane, it is incubated with the specific antibody and the second antibody labeled with HRP in turn, and then added with luminol luminescent reagent. If the target protein exists and binds with the antibody, a chemiluminescence reaction will occur, so that the location of the target protein is clearly visible. This method can not only improve the sensitivity of detection, but also ensure the accuracy of experimental results.
Desheng: Committed to the research and production of chemiluminescence reagents
Desheng is a company that has been focusing on the field of chemiluminescence reagents for many years. With its rich experience and professional team, it has successfully developed and produced a series of chemiluminescence reagents, including but not limited to luminol and acridine esters. These products not only meet the sensitive and safe detection needs of domestic and foreign markets, but also promote technological progress in related fields.
Desheng is well aware that high-quality luminescent reagents are the cornerstone of ensuring the success of experiments such as protein blotting. Therefore, the company continues to increase research and development investment, optimize production processes, in order to provide customers with specialized products. At the same time, Desheng also emphasizes cooperation and communication with research institutions, actively participates in the formulation of industry standards, and jointly promotes the development of biomedical research.
Product packaging
Conclusion
Luminol not only improves the sensitivity and safety of detection, but also provides scientists with more convenient research tools. In the future, as more companies like Desheng continue to explore and innovate, we believe that luminol and other chemiluminescence reagents will play an increasingly important role in life science research and make greater contributions to human health.
Contact details
Contact number
Address: C8, Guanggu United Science and Technology City, Ezhou City, Hubei Province
Fax：0711-3704 589
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