The role of TOOS

TOOS， N-Ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline sodium salt is a commonly used chromogenic substrate for biochemical analysis. In the glycated albumin assay kit, TOOS reagent mainly serves as a part of the detection reaction, participating in the generation of color changes and indirectly reflecting the concentration of glycated albumin in the sample. It combines with specific enzymes or chemical reactions to quantitatively analyze target substances based on the depth of color.

Working principle

1. Specific binding: In the process of measuring glycated albumin, specific enzymes need to be used to convert glycated albumin into a detectable form. For example, using proteases to cleave the non glycosylated portion of albumin, while the retained glycosylated albumin can be detected through subsequent steps.

2. Redox reaction: Next, introduce an oxidant that can react with the ketone group on glycated albumin, such as 4-aminoantipyrine (4-AAP). This oxidant will convert the ketone group into a quinone structure and generate hydrogen peroxide in the process.

3. Color reaction: TOOS, as a color substrate, reacts with hydrogen peroxide and 4-AAP generated by the above reaction under the catalysis of peroxidase (POD) to form a red quinone imine dye. The color intensity of the dye is directly proportional to the content of glycated albumin in the sample.

4. Light absorption measurement: Measure the absorbance value of the generated dye at a specific wavelength using a spectrophotometer, and calculate the specific concentration of glycated albumin in the sample based on the standard curve.

Advantages of TOOS

High sensitivity: TOOS has a high molar absorptivity, which means that even very small amounts of target substances can produce significant color changes, making the detection limit lower and suitable for microanalysis.

Good stability: TOOS itself is relatively stable and not easily affected by environmental factors such as temperature and pH changes, which helps ensure the consistency and reliability of experimental results.

Fast color development: Using TOOS can achieve faster color development speed, shorten the entire detection process time, and improve work efficiency.

Low background interference: The specific reaction between TOOS and the target substance reduces the possibility of non-specific binding, lowers background noise, improves signal-to-noise ratio, and ensures the accuracy of the results.

Wide applicability: TOOS is not only suitable for the determination of glycated albumin, but also for the detection of various other biomolecules, such as creatinine, uric acid, etc., demonstrating its wide range of applicability.

Easy to operate: TOOS based detection methods usually do not require complex equipment or technology, and can be easily completed in ordinary laboratories, with relatively low technical requirements for operators.

Product packaging

To sum up, TOOS plays an irreplaceable role in the saccharified albumin test kit (enzymatic method), providing researchers with an accurate and convenient detection method, which is of great significance for blood glucose control and monitoring of diabetes patients.

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