Is there a DNA type in addition to the RNA type of the Virus Transport Media?
Our Virus Transport Media is usually divided into two types, inactivated and non-inactivated Virus Transport Media, but both types are currently targeted at RNA viruses, mainly because the coronaviruses in the current epidemic are all RNA viruses. Viruses actually include DNA viruses and RNA viruses, so can the Virus Transport Media be suitable for DNA viruses?
The answer is that it cannot be used directly for DNA virus sampling, but can be applied after adjustment. Regardless of the type of Virus Transport Media, the ultimate goal is to protect the viral nucleic acid from rapid degradation in vitro and facilitate the amplification of specific nucleic acid sequences to achieve the purpose of detection. Therefore, the Rnase enzyme inhibitor in the RNA-type Virus Transport Media can be replaced with a Dnase enzyme inhibitor, so as to ensure that the viral DNA will not be degraded, thereby achieving the purpose of detection.
Both DNA and RNA are genetic materials that control all life activities, collectively referred to as nucleic acids. RNA viruses have a mutant type that far exceeds DNA viruses. What role do DNA and RNA play in genetic expression?
DNA, RNA and corresponding codon bases
DNA transcription RNA, RNA translation protein
In the intercellular phase, the chromosomes are no longer pulled by the spindle, they begin to loosen, and gradually unscrew. This is when DNA gradually comes into contact with the outside world, and when it comes into contact with RNA that paired with its base sequence, it will pair with it. This is the transcription process. After RNA transcription is completed, it will pass through the nuclear pore and into the cytoplasm together with hnRNP. Then RNA will meet the ribosome and begin to translate into protein.
The fate of DNA and RNA
Endonuclease cuts the DNA from the chromosome, leaves the nucleus with the cap of guanylic acid, and enters the cell sap. It is changed from double-stranded to single-stranded by helicase, and the nucleotide T is excised by DNA glycosidase. Short repair is performed by AP endonuclease (a protein enzyme that cuts off apurinic and apyrimidinic sites at the 5'end of DNA), DNA polymerase, and ligase. Such single strands have nucleotides unique to RNA. U will be gradually hydrolyzed when it encounters DNase. RNA is separated from the polyribosome and directly hydrolyzed by the RNase translated from the ribosome. Both DNA and RNA hydrolyzed nucleotides will enter the nucleus to start the next nucleic acid synthesis process.
In fact, both DNA and RNA are the basic materials that produce the miracle of life. Both perform their duties and are very similar. Both are involved in the expression of life genetic information. Viruses that only have protein and nucleic acid structures are only different in nucleic acids, so the corresponding Virus Transport Media They are also very similar, except that the enzymes that inhibit nucleic acid hydrolysis are different.
Trihydroxymethylaminomethane is an important laboratory reagent widely used in fields such as molecular biology and biochemistry. To ensure its quality and purity, a series of tests are required. This article provides measurement methods for appearance, content, solubility, drying weight loss, pH value, melting point, and UV absorbance. These methods help to evaluate the quality and purity of trimethylaminomethane.