The application of ESPMT (also known as TOPS, i.e. N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt) in creatinine detection kits is an important technological innovation in the field of biochemical detection. The following is a detailed analysis of its application in creatinine detection kits.

 

TOPS powder

 

1、Basic characteristics of ESPMT (TOPS)

 

ESPMT (TOPS) is a novel Trinder's colorimetric reagent with a unique chemical structure and properties. Its molecular structure contains functional groups such as sulfonic and amino groups, which can undergo oxidation reactions under specific enzymatic reaction conditions to generate color products with specific absorption spectra. The absorbance of this colorimetric product under a photometer is directly proportional to the concentration of creatinine, thus achieving quantitative detection of creatinine content.

 

2、The principle of ESPMT (TOPS) in creatinine detection

 

In creatinine testing, ESPMT (TOPS) is mainly combined with the creatine oxidase method (SOX method). The specific process is as follows:

 

Creatinine is hydrolyzed into creatine under the catalysis of creatine kinase (creatinine aminohydrolase).

 

Creatine is hydrolyzed into creatine and urea under the catalysis of creatine kinase (creatine amidine hydrolase).

 

Creatine is oxidized by sarcosine oxidase (SOX) to produce products such as hydrogen peroxide and formaldehyde.

 

Hydrogen peroxide reacts with ESPMT (TOPS) and 4-aminoantipyrine (4-AAP) under the catalysis of peroxidase (such as POD or HRP) to produce colorimetric products with specific absorption spectra.

 

The concentration of creatinine in the sample can be calculated by measuring the absorbance of the colorimetric product using a photometer.

 

3、Advantages of ESPMT (TOPS) in creatinine detection kit

 

High sensitivity: The combination of ESPMT (TOPS) and creatine oxidase method can significantly improve the sensitivity of detection, ensuring accurate detection results even at low concentrations of creatinine.

 

High stability: ESPMT (TOPS), as a chromogenic substrate, exhibits good stability during the reaction process, ensuring the accuracy and reliability of the detection results.

 

Easy to operate: The creatinine detection kit using ESPMT (TOPS) usually has the characteristics of easy operation and rapid response, which can greatly shorten the detection time and improve the detection efficiency.

 

Strong anti-interference ability: Some advanced creatinine detection kits also add other enzymes (such as ascorbate oxidase, catalase, etc.) to avoid the influence of interfering substances and further improve the accuracy of detection.

 

4、 Scope of application of ESPMT (TOPS) creatinine detection kit

 

The ESPMT (TOPS) creatinine detection kit is widely used in medical institutions for in vitro quantitative determination of creatinine levels in human serum, plasma, or urine. These test kits typically come with calibration standards and detailed operating instructions, ensuring the accuracy and traceability of test results.

 

Product packaging

 

5、 Summary

 

The application of ESPMT (TOPS) in creatinine detection kit not only improves the sensitivity and accuracy of detection, but also simplifies the operation process and shortens the detection time. Desheng is a supplier of diagnostic raw materials for the new Trinder's reagent. The ESPMT (TOPS) produced and developed by Desheng has gained unanimous recognition from many customers. With the continuous progress of medical technology and the continuous development of biochemical testing, the ESPMT (TOPS) creatinine detection kit will play an increasingly important role in clinical diagnosis.