SST coagulant tubes are commonly used as blood collection vessels for collecting, separating, and preserving serum samples. The coagulant and gel layer can promote the rapid coagulation of blood, separate serum and blood cells, and provide reliable samples for clinical testing and laboratory analysis. However, sometimes hemolysis may occur during the use of SST tubes for blood collection and separation, which can have a series of adverse effects on subsequent analysis. Below, we will discuss in detail the effects of hemolysis on everyone?

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Hemolysis phenomenon

Hemolysis, the process in which hemoglobin is released into the serum due to the rupture of red blood cells, may be caused by various factors, such as tube material, improper operation, and unsuitable centrifugation conditions. The occurrence of hemolysis during the serum separation process of SST coagulant tubes directly affects the quality of the serum.

The effect of SST coagulant tube hemolysis on serum quality

1. Hemolysis can lead to an increase in hemoglobin content in the serum, which can interfere with subsequent analysis and may also cause non-specific binding with other analytes, resulting in biased test results.

2. Hemolysis can cause changes in the levels of enzymes and electrolytes in the serum. Red blood cells contain various enzymes and electrolytes, and hemolysis can lead to the release of these substances into the serum, thereby altering the chemical composition of the serum. These changes may interfere with the determination of biochemical indicators, resulting in inaccurate results.

3. Hemolysis may also introduce the risk of microbial contamination. Red blood cell rupture may disrupt the natural barrier of the blood, making it easier for microorganisms to enter the serum, leading to contamination of serum samples during storage and transportation, which in turn affects subsequent analysis results.

The effect of hemolysis on the performance of SST coagulant tubes

The original intention of designing SST coagulant tubes was to improve the efficiency and effectiveness of serum separation. However, the occurrence of hemolysis may have adverse effects on this performance, with specific impacts as follows:

1. Hemolysis may affect the coagulation effect of SST coagulant tubes, and the main function of coagulants is to accelerate blood coagulation, thereby facilitating the separation of serum and blood cells. However, the phenomenon of hemolysis affects the effect of the coagulant, leading to insufficient serum separation or requiring longer coagulation time, thereby affecting the efficiency of the entire separation process.

2. The ideal serum separation process should be able to achieve the separation of serum and blood cells, avoiding cross contamination. However, hemolysis may cause red blood cell fragments or hemoglobin to enter the serum layer, reducing the purity of separation, which not only affects the accuracy of subsequent analysis, but may also cause contamination of experimental equipment.

Measures to deal with hemolysis in SST coagulant tubes

The occurrence of hemolysis not only affects serum quality and the performance of SST coagulant tubes, but may also have serious consequences for subsequent analysis. Therefore, a series of measures need to be taken to reduce the occurrence of hemolysis and its impact on analysis:

1. The operation process of blood collection and serum separation should be strictly controlled. During blood collection, excessive shaking and friction should be avoided to avoid damaging red blood cells. During the serum separation process, it should be ensured that the usage conditions of the SST coagulant tube meet the standards, such as temperature, humidity, etc. The control of centrifugal speed and time is also crucial and should be optimized according to the specific situation of the laboratory.

2. For samples that have already undergone hemolysis, strict screening and processing should be carried out. Before conducting analysis, it is possible to determine whether hemolysis has occurred by observing indicators such as serum color and transparency. For samples suspected of hemolysis, further validation and processing should be carried out to reduce the impact of SST coagulant tube hemolysis on analysis.

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3. We should strengthen the quality control and management of the laboratory. Regularly inspect and maintain experimental equipment, accelerators or separators, and consumables to ensure their stable and reliable performance. And a strict sample management system should be established to ensure that the sample collection, storage, and transportation processes comply with regulations.

Hemolysis during serum separation using SST coagulant tubes may have an impact on experimental results. Therefore, when collecting and processing samples, attention should be paid to operational details and reasonable control of centrifugation parameters. Only in this way can reliable experimental results be obtained. As a manufacturer of serum separation gel and coagulant, Desheng's product performance meets the standards and is the target of cooperation for many customers. At present, bulk spot sales are available with flexible gradient prices. If you have any relevant intentions, please click on the website to inquire about details and purchase!