The application of TOPS in creatinine detection

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As an important indicator of renal function testing, creatinine has always been a research hotspot in the field of clinical biochemical testing for its accurate and rapid measurement methods. With the development of biochemical technology, the emergence of new color reagents TOPS reagent provides a more reliable and efficient method for creatinine detection.


TOPS reagent


TOPS is a novel Trinder's reagent. The color development principle is based on the catalytic action of peroxidase (POD), which causes TOPS to react with hydrogen peroxide to generate red quinone imine compounds. The amount of red product generated is directly proportional to the concentration of creatinine, therefore, the absorbance of the red product can be measured indirectly by colorimetric method to determine the concentration of creatinine.


In practical applications, the use process of TOPS reagents is simple and efficient. Firstly, it is necessary to collect serum or plasma samples from patients and remove interfering substances. Then, mix the processed sample with creatinine detection reagents containing TOPS and perform the reaction under appropriate temperature and pH conditions. With the catalytic action of peroxidase, TOPS undergoes a colorimetric reaction with hydrogen peroxide, generating red quinone imine compounds. By measuring the absorbance of the reaction solution at a specific wavelength using a spectrophotometer and combining it with a standard curve, the creatinine concentration in the sample can be accurately calculated.


TOPS has shown many advantages in creatinine detection. Firstly, its high sensitivity enables accurate measurement results to be obtained even in low concentration creatinine samples. Secondly, TOPS reagents have good stability and are not easily affected by environmental factors, thus ensuring the accuracy and reliability of the detection results. In addition, TOPS reagents are not only suitable for creatinine detection, but can also be used for the determination of other biochemical indicators, such as uric acid, cholesterol, etc., which greatly expands their clinical application scope. The usage process of TOPS reagent is simple and clear, with convenient operation and rapid color reaction, which helps to improve detection efficiency.


With the continuous advancement of medical technology and innovation in biochemical detection methods, the application of TOPS in creatinine detection will be further optimized and improved. In the future, researchers will strive to improve the detection accuracy of TOPS reagents by optimizing their formulation and improving reaction conditions, further enhancing their sensitivity and accuracy in creatinine detection. Meanwhile, exploring the application of TOPS in the detection of other biochemical indicators will also become a research hotspot to meet the diverse needs of clinical diagnosis and treatment. In addition, with the continuous development of automation technology, combining TOPS reagents with automated detection equipment to achieve automation and intelligence in creatinine detection will help improve detection efficiency and quality. By optimizing production processes and reducing raw material costs, the production cost of TOPS reagents can be reduced, making them more widely used in clinical testing and providing accurate and efficient diagnostic services for more patients.


In summary, TOPS, as a new type of chromogenic reagent, has shown significant advantages and broad application prospects in creatinine detection. With the deepening of research and technological progress, TOPS will play an increasingly important role in the field of clinical biochemical testing, providing strong support for the diagnosis and treatment of diseases.


packing of products


Desheng specializes in producing over a dozen the new Trinder's reagents, including TOPS. After more than a decade of research and development, it can ensure that TOPS has a powder appearance, a purity of up to 99.5%, strong water solubility, and stable performance, ensuring the accuracy of experimental results. Having a foothold in the raw material market of in vitro diagnostic kits, it is highly trusted and supported by customers both domestically and internationally. If you have any relevant intentions, please click on the official website for consultation!