The application and importance of chromogenic substrate TOOS in medical testing

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With the continuous advancement of medical technology, various high sensitivity detection methods and reagents are gradually entering people's vision. Among them, the chromogenic substrate TOOS (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt) plays an important role in clinical biochemical detection as a novel Trinder's reagent. Its high water solubility, sensitivity, and stability make TOOS reagents widely used in multiple detection items such as blood glucose, uric acid, and kidney function.



TOOS powder


The basic principle of TOOS reagents


The main application principles of TOOS reagents are based on enzymatic reactions and color changes. Under specific reaction conditions, the target substance in the test sample interacts with the corresponding enzyme to produce hydrogen peroxide. Subsequently, hydrogen peroxide undergoes an oxidation-reduction reaction with TOOS under the combined action of 4-aminoantipyrine (4-AAP) and peroxidase (POD), producing a red quinone imine compound. This compound has a clear absorbance at a specific wavelength, and by measuring this absorbance value, the concentration of the target substance in the test sample can be calculated.


Characteristics of TOOS reagents


1. High water solubility: Compared with traditional chromogenic substrates such as phenol and aniline, TOOS reagents have higher water solubility, which makes them more convenient to prepare and use, and less prone to precipitation or crystallization.


2. High sensitivity: The color change of TOOS reagent is significant, and it can produce significant color reactions even at lower concentrations, thereby improving the sensitivity of detection.


3. High stability: TOOS reagents have stable chemical properties and are not easily affected by environmental factors, therefore they have a long shelf life during storage and use.


The application areas of TOOS reagents


1. Liver function testing: In liver function testing, TOOS reagents are commonly used to measure indicators such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum. The changes in these indicators can reflect the degree of liver cell damage and the status of liver function, providing a basis for liver diagnosis.


2. Blood glucose detection: TOOS reagents can also be used for blood glucose detection. Under the action of glucose oxidase, glucose is oxidized to gluconic acid and produces hydrogen peroxide. Subsequently, hydrogen peroxide reacts with TOOS reagent to generate red quinone imine compounds, thereby achieving quantitative determination of blood sugar.


3. Uric acid testing: Uric acid is an end product of purine metabolism in the body, and its concentration changes can reflect the status of renal function. Under the action of uricase, uric acid is hydrolyzed into allantoin and hydrogen peroxide. Subsequently, hydrogen peroxide reacts with TOOS reagent to generate red quinone imine compounds, thereby achieving quantitative determination of uric acid.


Precautions for using TOOS reagents


1. Sealed storage: Due to the reducibility of TOOS reagents, they are easily oxidized by factors such as oxygen in the air. Therefore, it is necessary to pay attention to sealed storage during use to avoid exposing the reagents to the air.


2. Ready to use: After preparing the TOOS reagent into a solution, it should be used as soon as possible to avoid prolonged exposure that may cause oxidation and discoloration of the solution.


3. Avoid contamination: During preparation and use, attention should be paid to avoiding contamination of the reagent by other substances, such as dust, to ensure the accuracy of the test results.



packing of products



With the continuous development of medical laboratory technology, the chromogenic substrate TOOS plays an increasingly important role in clinical biochemical testing due to its advantages of high sensitivity, stability, and water solubility.


Hubei Xindesheng Material Technology Co., Ltd. specializes in producing new types of Trinder's reagents. In addition to TOOS, there are also TOPS, ADOS, ADPS, etc. After more than a decade of dedicated research and development, the technology for producing The new Trinder's reagents has become very mature, and the products produced have also been exported to foreign countries. At present, there are over 400 domestic and foreign large, medium, and small new enterprises cooperating with Desheng, and their products and services are widely recognized by users. If you are also interested in the new Trinder's reagent, please click on the official website for consultation. Looking forward to communicating with you!