How to prepare 0.2mol/L Trihydroxymethylaminomethane buffer solution

Release time:


Tris base is a commonly used biochemical reagent widely used in the preparation of buffer solutions. In the laboratory, we often need to prepare Tris buffer solutions of different concentrations to meet experimental requirements. However, in most experiments, 0.1mol/L buffer solution was prepared, which is relatively less introduced compared to 0.2mol/L. So below, we will specifically discuss how to prepare a 0.2mol/L solution of Tris, including the required materials, steps, precautions, and possible applications.


Tris powder


Stores reserve

Tris powder: Ensure that you purchase high-quality Tris powder with a purity that meets the experimental requirements.

1. Distilled water or deionized water: used to dissolve trimethylaminomethane powder, ensuring that the quality of water meets experimental requirements.

2. Capacity bottle: Select a suitable capacity bottle, such as a 1L capacity bottle, for preparing and storing solutions.

3. Stirring rod or magnetic stirrer: used to accelerate the dissolution of trimethylaminomethane powder.

4. Electronic balance: used for weighing powders.

5. Label and pen: used to mark the prepared solution.


Preparation steps

1. Calculate the required mass of trimethylaminomethane powder: Calculate the required mass of powder based on the required solution concentration and volume of the volumetric flask. For a 0.2mol/L solution of trimethylaminomethane, the following formula can be used for calculation:

Mass (g)=Molar concentration (mol/L) x Volume of volumetric flask (L) x Molecular weight (g/mol)

The molecular weight of trimethylaminomethane is approximately 121.14g/mol. Therefore, for a 1L 0.2mol trimethylaminomethane solution, the required powder mass is approximately:

Mass=0.2mol/L × 1L × 121.14g/mol ≈ 24.23g

2. Weighing powder: Use an electronic balance to weigh the required powder, ensuring the accuracy of the balance and avoiding errors.

3. Dissolve trimethylaminomethane powder: Pour the weighed powder into a volumetric flask, add an appropriate amount of distilled or deionized water, and stir with a stirring rod or magnetic stirrer until the powder dissolves.

4. Constant volume: Continue to add distilled water or deionized water to the mark of the volumetric flask, ensuring that the solution volume is consistent with the nominal volume of the volumetric flask.

5. Shake well: Gently shake the volumetric flask to mix the solution evenly.

6. Marking: Attach a label to the volumetric flask, indicating information such as the name, concentration, and preparation date of the solution.


Matters needing attention

1. Accurate weighing: Use an electronic balance for weighing, do not estimate values to avoid errors.

2. Stir thoroughly: The powder needs to be dissolved to avoid impurities or undissolved particles in the solution.

3. Accurate volumetric determination: When adding distilled or deionized water to the mark on the volumetric flask, ensure that the solution volume is consistent with the nominal volume of the flask.

4. Avoid contamination: During the preparation process, attention should be paid to avoiding contamination of the solution, such as dust, bacteria, etc.

5. Storage conditions: The prepared solution should be stored in a cool, dry, and dark place, avoiding direct sunlight and high temperatures.


Product packaging


From the above, we know how to prepare a 0.2mol/L solution of trihydroxymethylaminomethane (Tris). The correct preparation method is beneficial for the smooth progress of the experiment, but this method is not uniform and sometimes needs to be flexibly adjusted according to experimental requirements. Desheng, as a manufacturer of bio buffering agent trimethylaminomethane, can provide high-quality analytical pure raw materials, with simple preparation and use, and provide technical guidance to solve problems in a one-stop manner. Welcome interested parties to click on the website to inquire about details and purchase!