The absorption wavelength of chromogenic substrate DAOS

Release time:

2024-02-17


The chromogenic substrate DAOS (4- (Dimethylamine) azobenzene-4 '- sulfonyl chloride) is a commonly used chromogenic reagent in the field of biochemistry, with an absorption wavelength of 593nm, belonging to the yellow light range. As a derivative of sodium aniline sulfonate, DAOS plays an important role in biochemical assay kits, especially in the application of Trinder's reagents, providing a sensitive detection method for biochemical experiments. Below, we will delve into the structure, spectral properties, and application of DAOS in biochemical assay kits.

DAOS powder

The structural characteristics of DAOS

The molecular structure of DAOS includes aniline and sulfonic acid groups, with the molecular formula C13H20NO6S and CAS number 83777-30-4. In this structure, the aniline group endows DAOS with the ability to absorb yellow light, while the sulfonic acid group enhances its water solubility. The uniqueness of the structure makes it more suitable for application in biochemical reagents.

The absorption wavelength characteristics of DAOS

The absorption wavelength of DAOS is located at 593nm, which belongs to the range of yellow light. This characteristic makes DAOS easy to measure in spectrophotometers, providing convenience for biochemical experiments. Yellow light wavelength has good transparency in biological and biochemical research, and has sensitivity and specificity for measuring many biomolecules. Therefore, the absorption characteristics of DAOS in the yellow light range make it an ideal choice for colorimetric reagents.

Application in Trinder's reagents

Trinder's reagents are a type of reagents widely used in biochemical detection, commonly used to detect physiological indicators in organisms, such as glucose and urea. The basic principle is to change the color of the substrate through enzymatic reaction of the chromogenic substrate, and then quantify the content of the target substance through absorbance measurement.

DAOS, as a chromogenic substrate in Trinder's reagents, reacts with substrates catalyzed by enzymes to generate colored products. The absorption wavelength of this colored product is located at 593nm, which is determined by the spectral properties of DAOS. This enables the spectrophotometer to accurately measure the concentration of reaction products, thereby achieving rapid and sensitive detection of target substance content in living organisms.

Factors affecting absorption wavelength

Although the main absorption wavelength of the chromogenic substrate DAOS is around 593nm, the following factors may affect its absorption wavelength:

1. Concentration: The concentration of DAOS may affect its absorption wavelength. At low concentrations, the absorption curve of DAOS may shift towards the long wavelength direction. Therefore, when using different concentrations of DAOS, attention should be paid to their potential impact on the experimental results.

2. PH: Under acidic or alkaline conditions, the absorption spectrum of DAOS will change, so it is necessary to maintain a stable pH value under experimental conditions.

3. Temperature: As the temperature increases, the absorption spectrum of DAOS may experience a redshift, meaning that the absorption wavelength shifts towards longer wavelengths. Understanding and controlling the temperature conditions during the experimental process can help improve the accuracy of the experiment.

4. Coexisting substances: Other substances in the experimental system may interact with DAOS, thereby affecting its absorption wavelength. For example, substances such as metal ions, organic solvents, and proteins may interact with DAOS, leading to changes in its spectral properties. Therefore, when selecting and using the chromogenic substrate DAOS, the influence of other substances that may exist in the experimental system on its spectral properties should be considered.

Product packaging

Due to its unique structure and absorption wavelength characteristics, the chromogenic substrate DAOS has been widely used in biochemical experiments, making it a sensitive, convenient, and reliable detection method. As a manufacturer of color developing substrates, Desheng can supply DAOS with high sensitivity, good solubility, and convenient operation, making it a choice for many people. If you have any relevant intentions, please click on the website to inquire about details and purchase!