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Oxidases and dehydrogenases in enzyme preparations: functional differences and application analysis

Oxidases and dehydrogenases in enzyme preparations: functional differences and application analysis

Although dehydrogenases and oxidases belong to oxidoreductases, they have core differences in electron acceptors and mechanisms. Dehydrogenases typically transfer hydrogen through cofactors such as NAD ⁺ and participate in cellular metabolism; And oxidase directly transfers electrons to oxygen molecules. This difference determines their different choices in biological functions and application scenarios.

2026-01-26

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The application value of Tricine (CAS: 5704-04-1) in the remediation of halogenated hydrocarbon pollution

The application value of Tricine (CAS: 5704-04-1) in the remediation of halogenated hydrocarbon pollution

Tricine (tris (hydroxymethyl) methylglycine) exhibits unique potential as a Good's buffer in halogenated hydrocarbon pollution remediation. It effectively improves degradation selectivity and electron utilization efficiency by stabilizing highly active carbene intermediates in dehalogenation reactions, reducing the generation of toxic by-products. This technology adopts an environmentally friendly in-situ injection method, which is suitable for complex site restoration and in line with the trend of green environmental protection development.

2026-01-25

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CAPSO: A Reliable Buffer Selection for Supporting High pH Biochemical Experiments

CAPSO: A Reliable Buffer Selection for Supporting High pH Biochemical Experiments

CAPSO, as an important Good's buffer, has a pKa of 9.6 and an effective buffering range of pH 8.9-10.3, making it particularly suitable for biochemical experiments that require alkaline environments. It effectively ensures the accuracy, reproducibility, and reagent performance of experiments in fields such as membrane protein extraction, immunoblotting, enzymatic reactions, and in vitro diagnostics by stabilizing pH, reducing ion interference, and metal complexation.

2026-01-24

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Application of Sodium 3- (N-morpholino) propanesulfonate in the Fermentation Culture of Epomycin

Application of Sodium 3- (N-morpholino) propanesulfonate in the Fermentation Culture of Epomycin

Ebomycin, as an anti-tumor macrolide, is mainly produced through biological fermentation. Among them, the buffer system is crucial for stabilizing the fermentation environment. 3- (N-morpholino) propanesulfonate sodium (MOPS Na), as a commonly used biological buffering agent, can effectively maintain the pH stability of the culture medium, reduce the impact of metabolic fluctuations on product synthesis, and thus ensure the stability and improvement of epomycin production.

2026-01-23

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Analysis of the key role of NHS groups in acridine ester labeling

Analysis of the key role of NHS groups in acridine ester labeling

The NHS group plays a crucial linking role in acridine ester chemiluminescence reagents. It can undergo specific reactions with primary amines on biomolecules such as antibodies and proteins, forming stable amide bonds, thereby achieving efficient covalent labeling of target molecules with acridine esters. The reaction conditions are mild and efficient, and should be carried out in a buffer system with pH 7.2-9, avoiding the use of buffer solutions containing primary amines to prevent interference. The linking ability of NHS esters is the basis for constructing stable labeling complexes.

2026-01-21

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Analysis of the causes of fogging when blood collection tube coagulant is sprayed on test tubes

Analysis of the causes of fogging when blood collection tube coagulant is sprayed on test tubes

The "misty film" on the inner wall of the blood collection tube is a natural result of the coagulant spraying process, formed by fine solid particles adhering to the tube wall after spraying and drying. The degree of haze mainly depends on the particle size of the coagulant raw material, and the larger the particle size, the more obvious the visual effect is usually. The uniform misty appearance is a direct reflection of good spray coating process control and can be used as a preliminary quality judgment, but the final quality still needs to be rigorously evaluated through laboratory performance testing.

2026-01-20

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