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Agarose Precast Gel Electrophoresis Kit
[Product Name]: Agarose Electrophoresis Kit
[English name]: Agarose Electrophoresis kit
[Contains ingredients]: 10 agarose precast gels, 15 mL of high-pressure fast electrophoresis solution, DNA loading buffer 6x 200µL, scissors and manual
[Specifications]: 8 holes 6 * 6, 6 holes 6 * 6, 13 holes 6 * 12, 18 holes 6 * 12, etc.
[Transport and storage]: Store and transport at 4 ° C, valid for 3 months, do not place below 0 ° C or above normal temperature, as the pre-made glue in the kit will freeze or deform, affecting your use.
[Self-provided reagents]: nucleic acid sample, Marker, deionized water
Product features and advantages:
1.You don't need to purchase reagents such as agarose, nucleic acid dyes, electrophoresis fluid and loading buffer, etc., all the kits are solved
2.This kit can save you about an hour of experiment time.
- Eliminate the tediousness of making your glue, and the pre-made glue is pre-stained with nucleic acid dye, no need for electrophoresis solution staining or post-staining. Simple and convenient, ready to use.
- This kit is specially equipped with high-pressure fast electrophoresis solution. If your nucleic acid sample fragment is below 2000bp, you can control the speed of electrophoresis at any time and adjust the voltage. The general mini gel can be completed in 5-10 minutes at the fastest; if The Marker or nucleic acid sample you used in the experiment has many bands and long fragments (nucleic acid sample fragments greater than 2000bp), you need to adjust to the appropriate low voltage according to the actual situation, or still use your original low-voltage electrophoresis conditions.
3.The DNA fragments obtained by electrophoresis of this product are used for gel recovery without affecting subsequent DNA ligation and other reactions.
1.Under low temperature conditions, crystals will precipitate out of the high-pressure fast electrophoresis solution. Please dissolve in a 65 ° C water bath and shake it evenly. Dilute with deionized water 100 times (1 mL of this product plus 99 mL of deionized water) for electrophoresis ,Pour the solution into the electrophoresis tank.
Voltage: If you are using an electrophoresis instrument that can set medium and high voltages, meet the conditions described in the above products and features 2, item b, and need to quickly produce results, for general minigels, you can electrophorese at 250-350V voltage 5-10 Minutes; for a large electrophoresis tank, it can be electrophoresed at 400-450V for 5-10 minutes. If the current or voltage gradually decreases during electrophoresis, please check whether the electrophoresis device has set a current upper limit or a power upper limit.
Repeated use: The electrophoresis solution prepared by this product can be reused at least 2-3 times. If a large electrophoresis tank is used, it can be used more times.
2.Take out a piece of individually packed agarose precast gel and cut it with scissors. The label side of the bag is the front side, or you can touch it by hand, and the hole protruding is the front side. Then take out the gel, with the front side facing up and the side of the hole as the negative electrode, put it in the high-pressure rapid electrophoresis solution, preferably 1mm without the glue surface, if there are bubbles in the sample hole, try to remove it.
3.Add 1µl of 6 × loading buffer to the DNA sample. After mixing, use a pipette to slowly add the sample mixture to the submerged gel sample well. Also add your own Marker.
4.Turn on the power, red is the positive electrode and black is the negative electrode. Remember that the DNA sample moves from the negative electrode to the positive electrode (the end near the sample hole is negative).
5.According to the position of the indicator swimming, determine whether to terminate the electrophoresis.
6.After electrophoresis is completed, turn off the power, observe the electrophoresis band and its position with a gel imager, and compare the size of the amplified product with Marker.