What are the solvent requirements for acridine ester (NSP-DMAE-NHS)?

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Acridine ester (NSP-DMAE-NHS) is a fluorescent reagent that contains an acridine ring structure within its molecule, which endows it with strong fluorescence properties. In fluorescence labeling experiments, acridine esters can react with specific functional groups in molecules to achieve fluorescence labeling of molecules. By labeling with acridine ester (NSP-DMAE-NHS), the visualization and quantitative analysis of biomolecules are achieved, further understanding the molecular processes in organisms, and therefore widely used in biological research. However, selecting a suitable solvent is crucial for the experimental results before and after using acridine ester for labeling.

1、 Solvent selection before labeling

Before labeling with acridine ester, due to the activation of its terminal carboxyl group by NHS, it is necessary to choose a non proton dry anhydrous solvent when dissolving acridine ester. Non proton solvents can effectively avoid acid base reactions with the carboxyl groups in acridine esters, thereby maintaining their activity. Solvents such as acetone, dimethyl sulfoxide (DMSO), and N, N-dimethylformamide (DMF) are usually considered suitable choices. During the dissolution process, it is necessary to ensure the purity and dryness of the solvent to avoid adverse effects on the activity of acridine ester.

2、 Selection of solvents after labeling

After labeling, amide or ester bonds are formed between the acridine ester and the labeled substance, and the stability of these bonds is generally not significantly affected by proton based solvents. Therefore, in labeled experiments, commonly used solvents such as water, methanol, ethanol, etc. can usually be selected. However, it should be noted that the active site of acridine ester itself is unstable in alkaline and oxidizing environments. Therefore, if experiments need to be conducted under alkaline or oxidizing conditions, corresponding non strongly alkaline and non oxidizing systems should be prepared based on the stability of the labeled substance to maintain the stability and activity of acridine ester.

In summary, acridine ester, as an important luminescent labeling reagent, plays a crucial role in the selection of solvents in experiments. Before labeling, non proton dry anhydrous solvents should be selected to maintain their activity. After labeling, commonly used solvents can be selected, but strong alkaline and oxidation conditions need to be avoided. The correct solvent selection will help ensure the accuracy and reliability of experimental results. As a supplier of acridine ester, Desheng can provide six different functional groups of acridine ester raw materials with high sensitivity and stable luminescence. If you have any questions, there is a dedicated person to answer and serve you. If you have any needs, welcome to inquire and order!