Basic knowledge of blood collection tube anticoagulant heparin sodium

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This article will explain the basic knowledge of heparin sodium as an anticoagulant for blood collection tubes. Heparin sodium is an acidic mucopolysaccharide containing a sulfate group. Due to its natural anticoagulant properties, it is often used as an additive for blood collection tubes to prevent blood coagulation. Heparin sodium acts as an anticoagulant by indirectly inhibiting the activity of thrombin. The mechanism is that after heparin sodium is combined with antithrombin 3, the structure of antithrombin 3 will be changed, and the activity will be activated. When combined with coagulation factors, it will inhibit coagulation factors. Antithrombin 3 can form a complex with thrombin, resulting in weakened thrombin effect. Heparin sodium accelerates this reaction thousands of times. After the effect of thrombin is weakened, the process of fibrinogen into fibrin will also be weakened, and the time of coagulation will be greatly prolonged.

What precautions should be taken during the use of heparin sodium as an anticoagulant for blood collection tubes?

First of all, as  blood collection tube additives, refined and purified heparin sodium needs to be used, and its appearance is white powder. Because the crude product and purified heparin sodium have different test index data, the use of crude product may lead to inaccurate test results.

Secondly, when heparin sodium is used as a blood collection tube additive, it should be prepared and used immediately. When it is used, it needs to be prepared into a solution with deionized water or distilled water, but be careful not to leave it for too long, because heparin sodium is a mucopolysaccharide substance. It is easy to grow bacteria if it is not used for a long time after the solution is formed. The usual dosage of heparin sodium is 1mL of blood corresponding to 20IU of heparin sodium, so that better anticoagulation effect can be achieved.

Heparin exists in mammalian organs, and heparin sodium is generally extracted from animal livers. The most commonly used method for extracting heparin sodium in China is to extract pig small intestine. Because the domestic pig industry is very developed, the supply source of pig small intestine is very wide and it is easier to ensure the freshness of the goods. Desheng Biochemical independently develops and produces heparin sodium, heparin lithium and other anticoagulants. It has rich industry experience and strong technical strength. Friends who have questions and needs about heparin sodium can contact Desheng customer service. Products you need.