How does the nucleic acid release agent crack and inactivate the virus
Release time:
2020-12-31
The nucleic acid release agent can lyse the cell or virus protein coat, release the nucleic acid in it, and then perform the nucleic acid detection by PCR experiment. It has different types, among which the inactivated Virus Transport Mediais a nucleic acid release agent commonly used in the detection of new crowns.
Nucleic acid tests are often done in some places, and this inactivated Virus Transport Mediais also directly called a nucleic acid release agent. Its most fundamental feature is that it can quickly inactivate the virus, degrade the viral protein membrane capsid, and simultaneously degrade the viral nucleic acid. (The new crown is RNA) is released, so that PCR amplification experiments after reverse transcription can be performed to achieve the purpose of using nucleic acid to detect viruses.
How the nucleic acid release agent cleavage and inactivate the virus
There are many ways to split the virus:
1. Heating method: It usually needs to be heated at 80 to 100 degrees Celsius for 5-10 minutes. The operation is very simple. It only takes one step to obtain the template for nucleic acid PCR amplification. It does not require any centrifugation, extraction and other operating steps, which is more common than usual The CheleX100 extraction method and the phenol/chloroform method are fast. Although this method is simple and fast, the amount of nucleic acid extracted is small.
2. Concentrated salt method: High concentration of salt can destroy the secondary bond between nucleic acid and protein, disassociate nucleoprotein and release viral nucleic acid. Usually, guanidine hydrochloride, guanidine isothiocyanate or non-guanidine salt cracking salt can be used to directly lyse and inactivate the virus without heating the sample, which is simple and fast.
In addition to cleavage components, nucleic acid release agents usually contain detergents such as SDS that denature proteins and destroy membrane structure, biological buffers such as Tris, and inhibitors that prevent nucleases from degrading nucleic acids such as EDTA, and reagents such as NaCl to maintain the stability of nucleic acid structure. The lysis system may also add protease to cut the protein into small fragments to promote the separation of protein and nucleic acid.
Nucleic acid release agent or inactivated Virus Transport Mediais one of Desheng’s main products. In addition, in order to meet different testing needs, there are activated virus transport media. The products are compared in terms of customer feedback at home and abroad and factory supply capacity. ideal.
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