The entanglement between bicine and metal ions

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In most cases, metal elements do not exist in organisms in the form of white ions, but often form metal complexes with biological macromolecular ligands such as proteins and nucleic acids. The basic structural unit of protein is amino acid, and N,N-bis(2-hydroxyethyl)glycine (Bicine) first synthesized by KiplranoV in 1926 can be regarded as an analog of helium acid (glycine). As can be seen from the structure of Bicine, Bicine has the properties of both amino acid and aminoethanol. It has a substituted amino group, a shuttle group and two hydroxyl groups as coordinating groups, which can form complexes with metal ions. The most basic metal ion bonding site in Bicine molecule is its amino acid part.

In 1966, Good et al. found that Bicine has a pH range of 7.6-9.0, which is suitable for hydrogen ion buffering in biological systems. It is easily soluble in water, insoluble in acetone, DMF dimethyl formamide, DMSO dimethyl sulfoxide, Organic solvents such as DMAc dimethylacetamide; the aqueous solution (25% concentration) is colorless and transparent, has a small salt effect and is difficult to penetrate biofilms. Therefore, Bicine is often referred to as "Good'S" buffer. However, these pH buffers may interact with metal ions by forming complexes in solution, which makes some research results effective only when the buffer is at a certain concentration. For example, when calculating the binding constant of protein and metal ions, if you do not consider the role of metal ions and buffers, you will get wrong conclusions. Therefore, the assumption that Bicine has little or no interaction with metal ions when used as a buffer is unreasonable. Many experimental facts have proved that Bicine and metal can form stable binary and ternary complexes. The stability of these complexes in solution is widely studied.

Therefore, the interaction between Bicine and metal ions has attracted more and more attention. The fact that Bicine can form binary and ternary complexes reminds us to use Bicine as a buffer when there are metal ions and biological ligands that may coordinate. Agents should be particularly cautious. In addition, since Bicine is coordinated with metal ions, its two hydroxyl groups are weaker coordination groups, so when there are other ligands with stronger coordination ability, it is easy to form mixed complexes. Moreover, the metabolic reaction in the organism involves the balance between a variety of metal ions and a variety of donor molecules. In vitro studies on the balance of transition metal ions and two or more ligands are helpful to accurately interpret the in vivo Cooperation phenomenon. Therefore, the interaction between the amino acid analogue Bicine and transition metal complexes is an area to be further studied.

Bicine produced by Desheng has a purity of ≥99% and a chloride ion content of ≤0.1% (chloride ion content can also be customized according to requirements), good water solubility, stable process, and can ensure the appearance of the product is pure white crystal powder.