The role of virus inactivation transport media in the nucleic acid detection process of new coronavirus
At present, the conventional detection method for new coronavirus infection is nucleic acid determination (real-time fluorescent RT-PCR method), and nucleic acid detection is the gold standard for the diagnosis of new coronary pneumonia. The result of nucleic acid detection is an important basis for clinical diagnosis and rehabilitation of patients with new coronary pneumonia, so what process does this result go through? What role does the virus inactivation transport media play in it?
Virus inactivation transport media
Because the new coronavirus has strong infectivity and specificity, the nucleic acid detection process is extremely dangerous. According to the "Guidelines for Laboratory Testing of New Coronavirus Infected Pneumonia" (Second Edition) issued by the National Health and Health Commission, the detection of any new coronavirus must be carried out in a laboratory with appropriate conditions and personnel trained in relevant technical safety operating.
New Coronavirus nucleic acid detection process:
Step 1: Receive samples
The nucleic acid detection process starts with the detection personnel receiving samples. Before receiving the sample, the testing personnel should wear according to the three-level protection standard, wear protective clothing, goggles, isolation clothing, and wear N95 masks, double gloves, put on foot covers, etc.
Then use 75% ethanol to sterilize the space and countertop of the biosafety cabinet in the laboratory and the nucleic acid extraction instrument, etc., also need to sterilize the specimen transfer box, and check the name, gender, age, number and test information of the sample being tested Wait, after confirming that the transfer box is intact, place it in the biological safety cabinet.
The second step: virus inactivation
Virus inactivation is a very important step. It is usually inactivated with a 56° water bath for about 30 minutes. This process can reduce the virus toxicity, greatly reduce the risk of infection of the operator, and will not affect the test results. After the virus is inactivated, in order to prevent the aerosol from contaminating the laboratory and testing personnel after opening the cover, it is necessary to stand at room temperature for some time.
Step 3: Nucleic acid extraction
Nucleic acid extraction is the most dangerous step, and the sample needs to be carefully opened in the biosafety cabinet of the negative pressure laboratory. Then add nucleic acid extraction reagents to destroy the virus cells and let the nucleic acids be released.
Step 4: Nucleic acid analysis, waiting for the result
In the biological safety cabinet, add the extracted nucleic acid to the PCR amplification reaction system, and then put the constructed amplification system into the amplifier to perform nucleic acid analysis.
Step 5: Disinfection, waste disposal
After the completion of a series of steps, it is necessary to dispose of the experimental waste and disinfect the experimental instruments, desktop, and ground.
The most dangerous of the above detection steps is nucleic acid extraction, because the new coronavirus is highly infectious and has many routes of transmission. Is there any way to solve this problem? The virus inactivation transport media overcomes this difficulty very well. Virus inactivation transport media can quickly inactivate and store respiratory pathogens by using lysed salt, so that the samples lose their infectivity. Because it is an inactivated virus, there is no need to cultivate the virus. The first thing is to cleave and inactivate the virus and destroy its membrane protein to release nucleic acid, which greatly reduces the risk of nucleic acid extraction.
Desheng can provide virus-inactivated and non-inactivated Virus Transport Medias for different needs, as well as nasal and pharyngeal swabs for collecting specimens.
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.